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07-1229 Anti-DAPK2 Antibody

07-1229
100 µg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information
Replacement Information07-1229 is a recommended replacement for AB3606.

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1229
ReplacesAB3606
DescriptionAnti-DAPK2 Antibody
Alternate Names
  • DAP kinase
  • DAP- kinase-related protein
  • Death-associated protein kinase
Background InformationDeath Associated Protein Kinase (DAPK) is a Ca2+/Calmodulin-dependent, cytoskeletal associated protein kinase whose expression is implicated in the sensitivity of cells to apoptotic effects of TNFα and Interferon-γ. Deletion of the Calmodulin-binding domain results in a constitutively active mutant with a more potent cytotoxic effect. In contrast, the catalytically inactive mutant exerts a dominant negative effect, reducing cytotoxicity and protecting cells from Interferon-γ induced cell death. DAPK expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlate with high rates of metastasis.
Product Information
FormatAffinity Purified
Control
  • C2C12 cell lysate
PresentationPurified rabbit polyclonal IgG in buffer containing PBS and 0.02% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect DAPK2 using this Anti-DAPK2 Antibody validated for use in WB, IH(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemisty (paraffin): DAPK2 representative staining pattern/morphology in apoptotic bodies in colon cancer. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB.

Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
Biological Information
ImmunogenPeptide corresponding to aa 356-370 (ARRKALHPRRRSSTS) of human DAPK2, which is identical to the sequence of mouse origin.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityDetects a 42 kDa band by western blot, corresponding to DAPK2. Does not cross-react with DAPK1.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites.
Gene Symbol
  • DAPK2
  • DRP-1
  • MGC119312
  • EC 2.7.11.1
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9UIK4 # Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.
COFACTOR: Magnesium.
SIZE: 370 amino acids; 42898 Da
SUBUNIT: Homodimer. Homodimerization is required for apoptotic function and is inhibited by autophosphorylation at Ser-318.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Ubiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle.
SIMILARITY: SwissProt: Q9UIK4 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. & Contains 1 protein kinase domain. ...
Molecular WeightDetects a 42 kDa band by western blot.
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on C2C12 lysates.

Western Blot Analysis:
1:500-1,1000 dilution of this lot detected DAPK2 on 10 μg of C2C12 lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at 2-8° C from date of receiept.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
07-1229 04053252367700