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CC12 Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6)

This Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of Cyclin D1 (Ab-3).

Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Bcl-1, Anti-PRAD-1

CC12
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
CC12-100UG Plastic ampoule 100 μg
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Description
OverviewRecognizes cyclin D1 in MCF-7 and A431 cells. Does not react with cyclins D2 and D3.
Catalogue NumberCC12
Brand Family Calbiochem®
SynonymsAnti-Bcl-1, Anti-PRAD-1
References
ReferencesInaba, T., et al. 1992. Genomics 13, 565.
Jiang, W., et al. 1992. Cancer Res. 2, 2980.
Pines, J. 1992. Cell Growth Differ. 2, 305.
Xiong, Y., et al. 1992. Genomics 13, 575.
Xiong, Y., et al. 1992. Cell 71, 504.
Motokura, T., et al. 1991. Nature 350, 512.
Solomon, M.J., et al. 1990. Cell 63, 1013.
Draetta, G., and Beach, D. 1988. Cell 54, 17.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Negative controltrp E (Ab-1)
Positive controlMCF7, or A431 cells or breast carcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting, Immunoprecipitation, and Neutralization Studies Lukas, J., et al. 1994. Oncogene 9, 707. Immunofluorescence and Flow Cytometry Bartkova, J., et al. 1995. Cancer Res. 55, 949-956. Bartkova, J., et al. 1994. J. Pathol. 172, 237-245. Lukas, J., et al. 1994. Oncogene 9, 707. Paraffin and Frozen Sections Bartkova, J., et al. 1995. Cancer Res. 55, 949-956. Bartkova, J., et al. 1994. J. Pathol. 172, 237-245. Gillett, C., et al. 1994. Cancer Res. 54, 1812-1817.
Key Applications Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Neutralization Studies
Paraffin Sections
Application NotesFlow Cytometry (5 µg/ml; Cat. No. CC12, CC12F)
Frozen Sections (see application references)
Immunoblotting (1 µg/ml)
Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F)
Immunoprecipitation (see application references)
Neutralization Studies (see application references)
Paraffin Sections (5 µg/ml, heat pre-treatment required)
Application CommentsDoes not react with cyclins D2 or D3. Staining of paraffin sections can also be detected with pepsin or trypsin pretreatment; light staining will be detected without pretreatment. Do not microwave sections, as this may destroy staining. For immunofluorescence and flow cytometry, we recommend using the FITC conjugate (Cat. No. CC12F). Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant human cyclin D1
ImmunogenHuman
CloneDCS-6
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Catalogue Number GTIN
CC12-100UG 04055977228465