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ABS1645 Anti-Chromatin-Modifying Protein 6

Anti-Chromatin-Modifying Protein 6, Cat. No. ABS1645, is a rabbit polyconal antibody that is used to detect Chromatin-Modifying Protein 6 and has been tested for use in Immunofluorescence and Western Blotting.

Anti-Chromatin-Modifying Protein 6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: CHMP6, VPS20 UniProt Number: Q96FZ7
ABS1645
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MkWB, IFRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABS1645
DescriptionAnti-Chromatin-Modifying Protein 6
Alternate Names
  • Chromatin-modifying protein 6
  • Charged multivesicular body protein 6
  • Vacuolar protein sorting-associated protein 20
  • Vps20
  • hVps20
Background InformationCharged multivesicular body protein 6 (UniProt: Q96FZ7; also known as Chromatin-modifying protein 6, Vacuolar protein sorting-associated protein 20, Vps20, hVps20, CHMP6) is encoded by the CHMP6 (also known as VPS20) gene (Gene ID: 79643) in human. CHMP6 is a human orthologue of yeast Vps (vacuolar protein sorting) 20, a component of ESCRT (endosomal sorting complex required for transport)-III. Various CHMP6 orthologues in organisms ranging from yeast to humans contain the N-myristoylation consensus sequence at each N-terminus. CHMP6 is an ubiquitously expressed protein that has an acidic C-terminus and the basic N-terminus that render the protein in a closed, soluble and inactive conformation through an auto-inhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components. CHMP6 is probable core component of the endosomal sorting required for transport complex III (ESCRT-III), which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Chromatin-Modifying Protein 6, Cat. No. ABS1645, is a rabbit polyconal antibody that is used to detect Chromatin-Modifying Protein 6 and has been tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesImmunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Chromatin-Modifying Protein 6 in Cos-7 cells (Langelier, C., et. al. (2006). J Virol. 80(19):9465-80).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Chromatin-Modifying Protein 6 in HEK293 cell lysate (Langelier, C., et. al. (2006). J Virol. 80(19):9465-80).
Biological Information
ImmunogenGST-tagged full length recombinant human Chromatin-modifying protein 6.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects chromatin-modifying protein 6 (CHMP6) in human and monkey cells.
IsotypeIgG
Species Reactivity
  • Human
  • Monkey
Species Reactivity NoteHuman, Monkey.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CHMP6
  • VPS20
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~32 kDa observed; 23.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Chromatin-Modifying Protein 6 in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS1645 04054839201370