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ABS2236-100UG Anti-CLSTN3b

Anti-CLSTN3b, Cat. No. ABS2236, is a rabbit polyclonal antibody that detects Calsyntenin 3b (Clstn3) and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

ABS2236-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABS2236-100UG
DescriptionAnti-CLSTN3b
Background InformationCalsyntenin 3beta (UniProt: A0A4P8DYG6; also known as Calsyntenin-3β, CLSTN3β) is encoded by the Clstn3 (also known as Clstn3b) gene (Gene ID: 232270) in murine species. CLSTN3β is a mammal-specific that is preferentially expressed in thermogenic adipocytes and is localized in endoplasmic reticulum. Its expression in brown adipose tissue is about six-fold higher than both the inguinal subcutaneous and the perigonadal visceral white adipose tissue. However, its expression can be induced in the inguinal subcutaneous white adipose tissue upon cold exposure. Brown adipose tissue thermogenesis require innervation by the sympathetic nerves that release norepinephrine, which activates β-adrenergic receptors-cAMP-PKA pathway to drive lipolysis and thermogenic respiration. CLSTN3β is shown to promote S100b protein release that promotes this innervation and its loss is shown to reduce functional sympathetic innervation in adipose tissue. Loss of CLSTN3β also predisposes mice to obesity on a high fat diet. Clstn3β knockout mice exhibit significantly higher fasting glucose levels, impaired glucose tolerance, more lipid accumulation in brown adipose tissue, and liver steatosis. (Ref.: Plucińska, K., et al. (2020). Front. Endocrinol. 11; 579785; Zeng, X., et al. (2019). Nature. 569(7755); 229-235).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in PBS with 0.05% Sodium Azide
Quality LevelMQ200
Applications
ApplicationAnti-CLSTN3b, Cat. No. ABS2236, is a rabbit polyclonal antibody that detects Calsyntenin 3b (Clstn3) and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunofluorescence
Application NotesTested Applications

Evaluated by Western Blotting in lysate from wild-type brown adipocytes.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Calsyntenin 3b (Clstn3) in lysate from wild-type adipocytes, but not in lysate from Calsyntenin 3b knock out adipocytes.

Immunoprecipitation Analysis: A representative lot immunoprecipitated CLSTN3b in Immunoprecipitation applications (Zeng, X., et. al. (2019). Nature. 569(7755): 229-235).

Western Blotting Analysis: A representative lot detected CLSTN3b in Western Blotting applications (Zeng, X., et. al. (2019). Nature. 569(7755): 229-235).

Immunofluorescence Analysis: 10 ug/mL from a representative lot detected CLSTN3b in mouse primary brown adipocytes (Courtesy of Dr. Xing Zeng @UT Southwestern Medical Center).

Immunofluorescence Analysis: A representative lot detected CLSTN3b in Immunofluorescence applications (Zeng, X., et. al. (2019). Nature. 569(7755): 229-235).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of murine Calsyntenin 3b.

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EpitopeC-terminal
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects murine Calsyntenin 3b. It targets an epitope within 18 amino acids from the C-terminal region.
IsotypeIgG
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Clstn3
  • Clstn3b
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~27 kDa observed; 39.56 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from wild-type brown adipocytes.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Calsyntenin 3b (Clstn3) in lysate from wild-type adipocytes, but not in lysate from Calsyntenin 3b knock out adipocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS2236-100UG 04065266298246