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MABS2280-25UG Anti-CLEC18A Antibody, clone 3F10F

Anti-CLEC18A, clone 3F10F, Cat. No. MABS2280, is a mouse monoclonal antibody that detects CLEC18A and is tested for use in ELISA, Flow Cytometry, and Western Blotting.

MABS2280-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS2280-25UG
DescriptionAnti-CLEC18A Antibody, clone 3F10F
Alternate Names
  • C-type lectin domain family 18 member A
  • Mannose receptor-like protein 2
Background InformationC-type lectin domain family 18 member A (UniProt: A5D8T8; also known as Mannose receptor-like protein 2) is encoded by the CLEC18A (also known as MRLP2) gene (Gene ID: 2348174) in human. CLEC18 is a novel secretory lectin that is expressed in all cells but is abundantly expressed in hepatocytes and peripheral blood cells. It is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off to generate the mature form (aa 27-446). Its C-type lectin domain is localized in amino acids 306-433. CLEC18 is mainly localized in the endoplasmic reticulum, Golgi apparatus, and endosome. Although it is expressed in normal hepatocytes, it is absent in hepatocellular carcinoma. It binds polysaccharides in a Ca2+-independent manner and shows preferential binding to fucoidal, β-glucans and galactans. It also serves as a co-receptor for TLR3 and contributes to the differential immune responses to poly(I:C) and H5N1 influenza A virus infection. Its levels are shown to be up-regulated upon differentiation of monocytes into macrophages and dendritic cells. Significantly higher levels of CLEC18A are observed subjects with hepatitis C infection and this increased level is associated with increased cryoglobulin levels, which may be related to the occurrence of mixed cryobulinemia (MC) syndrome. (Ref.: Huang, YL., et al. (2021). Nat. Commun. 4;229; Liao, TL., et al. (2018). Sci. Rep. 8; Article 17287; Huang, YL., et al. (2015). J. Biol. Chem. 290(35); 21252-21263).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-CLEC18A, clone 3F10F, Cat. No. MABS2280, is a mouse monoclonal antibody that detects CLEC18A and is tested for use in ELISA, Flow Cytometry, and Western Blotting.
Key Applications
  • Western Blotting
  • Flow Cytometry
  • ELISA
Application NotesTested Applications

ELISA Analysis: A representative lot detected CLEC18A in ELISA applications (Liao, T-L., et al. (2018). Sci Rep. 8(1); 17287).

Flow Cytometry Analysis: A representative lot detected CLEC18A in Flow Cytometry applications (Liao, T-L., et al. (2018). Sci Rep. 8(1); 17287).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis/Sumo-tagged full-length recombinant mature C-type lectin domain family 18 member A (CLEC18A) (without the signal peptide).
EpitopeUnknown
Clone3F10F
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 3F10F is a mouse monoclonal antibody that detects C-type lectin domain family 18 member A (CLEC18A).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CLEC18A
Purification MethodProtein G purified
UniProt Number
Molecular Weight~58 kDa observed; 49.6 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant Human CLEC18A protein.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant Human CLEC18A protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2280-25UG 04065268527498