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AB5613 Sigma-AldrichAnti-Brain Derived Neurotrophic Factor Antibody, pro

Anti-Brain Derived Neurotrophic Factor Antibody, pro is an antibody proBDNF for use in Immunohistochemistry and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References

Research Category: Neuroscience Research Sub-Category: Neurochemistry & Neurotrophins, Neuroinflammation & Pain Gene Symbol: BDNF UniProt Number: P23560

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	IHC, WB	Rb	Serum	Polyclonal Antibody

Description
Catalogue Number	AB5613
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Brain Derived Neurotrophic Factor Antibody, pro
Alternate Names	Prepro-Abrineurin Pro-Abrineurin Prepro-BDNF Pro-BDNF Prepro-Brain-derived neurotrophic factor Pro-Brain-derived neurotrophic factor
Background Information	Brain-derived neurotrophic factor (UniProt P23560; also known as Abrineurin, BDNF, Neurotrophin) is encoded by the BDNF (also known as BULN2) gene (Gene ID 627) in human. BDNF is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT3, and NT4/5. BDNF mediates its neurotrophic properties by signaling through the high affinity cell surface receptor gp145/trkB. BDNF is initially produced with a signal peptide (a.a. 1-18) and a propeptide (a.a. 19-128) sequence, the removal of which yields the mature BDNF (a.a. 129-247).

References

Product Information
Format	Serum
HS Code	3002 15 90
Presentation	Rabbit serum. Lyophilized. Reconstitute with 50 μL of sterile distilled water.
Quality Level	MQ100

Applications
Application	Anti-Brain Derived Neurotrophic Factor Antibody, pro is an antibody proBDNF for use in Immunohistochemistry and Western Blotting.
Key Applications	Immunohistochemistry Western Blotting
Application Notes	Western blot: 1:1,000-1:5,000 Immunohistochemistry: 1:1,000-1:5,000 Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to a region within human BDNF propeptide sequence.
Epitope	Propeptide.
Host	Rabbit
Specificity	This polyclonal antiserum detects prepro- and pro-BDNF, as well as prepro-/pro-BDNF-derived N-terminal fragments that contain the propeptide sequence (a.a. 19-128), but not mature BDNF or C-terminal fragments without the propeptide sequence. No cross-reactivity to other proneurotrophins.
Species Reactivity	Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_001709 NM_170731 NM_170734 NM_001143810 NM_001143809
Gene Symbol	BDNF
UniProt Number	P23560
Molecular Weight	~35 kDa (glycosylated prepro-/pro-BDNF), ~50-65 kDa (glycosylated prepro-/pro-BDNF dimer), and ~26-28 kDa (non-glycosylated prepro/pro-BDNF or glycosylated prepro-/pro-BDNF N-terminal fragments) observed. Target bands appear larger than the calculated molecular weights of 27.82/28.87/29.82/37.12/31.12 kDa (human prepro-BDNF isoform 1/2/3/4/5) and 25.77/26.82/27.77/35.07/29.06 kDa (human pro-BDNF isoform 1/2/3/4/5) due to glycosylation and/or dimerization. Uncharacterized band(s) may appear in some lysates.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C undiluted for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (ACS grade or better) can be added (1:1) for additional stability.

Packaging Information
Material Size	50 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
AB5613	04053252274855

Documentation

Anti-Brain Derived Neurotrophic Factor Antibody, pro SDS

Title
Safety Data Sheet (SDS)

Anti-Brain Derived Neurotrophic Factor Antibody, pro Certificates of Analysis

Title	Lot Number
RABBIT ANTI-proBDNF - 2539973	2539973
RABBIT ANTI-proBDNF - 3194638	3194638
RABBIT ANTI-proBDNF -2510130	2510130
RABBIT ANTI-proBDNF -2578093	2578093
RABBIT ANTI-proBDNF -2615698	2615698
RABBIT ANTI-proBDNF -2661300	2661300
RABBIT ANTI-proBDNF -2701577	2701577
RABBIT ANTI-proBDNF -2707205	2707205
RABBIT ANTI-proBDNF POLYCLONAL ANTIBODY - 2074748	2074748
RABBIT ANTI-proBDNF POLYCLONAL ANTIBODY - 2506277	2506277

References

Reference overview	Pub Med ID
Progesterone treatment alters neurotrophin/proneurotrophin balance and receptor expression in rats with traumatic brain injury. Milos Cekic,Sarah J Johnson,Vinay H Bhatt,Donald G Stein Restorative neurology and neuroscience 30 2012 Show Abstract The neuroactive steroid progesterone (PROG) has been shown to be an effective treatment for traumatic brain injury (TBI) both in animal models and in humans, but the signaling pathways involved have not yet been fully described. Here we characterize the protein expression of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and their pro-proteins and receptors following PROG treatment for TBI.	22232032

Reference overview

Pub Med ID

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