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MABC1022 Anti-BCL2L12 Antibody, clone 11B5.1

Detect Bcl2L12 using this mouse monoclonal Anti-BCL2L12, clone 11B5.1 Antibody, Cat. No. MABC1022, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-BCL2L12 Antibody, clone 11B5.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: BCL2L12, BPR UniProt Number: Q9HB09
MABC1022
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1022
DescriptionAnti-BCL2L12 Antibody, clone 11B5.1
Alternate Names
  • Bcl-2-like protein 12
Background InformationBcl-2-like protein 12 (UniProt Q9HB09; also known as Bcl-2-related proline-rich protein, Bcl2-L-12) is encoded by the BCL2L12 (also known as BPR) gene (Gene ID 83596) in human. Bcl-2 family proteins paly either a pro- or anti-apoptotic role in cell death signaling. BCL-2 family members are related by regions of sequence and structural homology. The pro-survival members contain four BCL-2 homology regions (BH1 BH4), whereas the BH3-only members contain only the BH3 amphipathic helix that mediates their interaction with the groove of multi-domain BCL-2 family members. Bcl2-L-12 is an anti-apoptotic protein that shares a lower amino acid similarity with the other Bcl2 family members. It contains a BH2 (a.a. 311-322) domain and a BH3-like domain (a.a. 192-220) that mediate its interaction with Bcl2 and Bcl-xL. Exogenous expression of Bcl2-L-12 with Leu213 or Leu217 mutation in U87MG cell line is reported to result in reactivation of PARP cleavage, caspase-3 and cytochrome c release compared to cells expressing wild-type Bcl2-L-12.
References
Product Information
FormatPurified
PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Bcl2L12 using this mouse monoclonal Anti-BCL2L12, clone 11B5.1 Antibody, Cat. No. MABC1022, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected BCL2L12 in human pancreas, skeletal muscle, and tonsil tissue sections.
Biological Information
ImmunogenGST-tagged recombinant human BCL2L12 internal fragment.
Clone11B5.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 11B5.1 targets an internal epitope present in spliced isoforms 1&3, but absent in isoform 2, of human BCL2L12 reported by UniProt (Q9HB09).
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • BCL2L12
  • BPR
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~37 kDa observed. 36.82/36.67 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in PC3 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected BCL2L12 in 10 µg of PC3 human prostate cancer cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1022 04054839057199