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MABE1138-25UL Anti-BAF180 Antibody, clone 370.1.8C3

Anti-BAF180, clone 370.1.8C3, Cat. No. MABE1138, is a highly specific rat monoclonal antibody that detects Protein Polybromo-1 (BAF180) and has been tested for use in Western Blotting.

Anti-BAF180 Antibody, clone 370.1.8C3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: PBRM1, BAF180, PB1 UniProt Number: Q86U86
MABE1138-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1138-25UL
DescriptionAnti-BAF180 Antibody, clone 370.1.8C3
Alternate Names
  • Protein polybromo-1
  • hPB1
  • BRG1-associated factor 180
  • Polybromo-1D
Background InformationProtein polybromo-1 (UniProt: Q86U86; also known as hPB1, BRG1-associated factor 180, BAF180, Polybromo-1D) is encoded by the PBRM1 (also known as BAF180, PB1) gene (Gene ID: 55193) in human. BAF180 is a large, chromatin-remodeling protein that contains six bromodomains, two BAH-domains, and one HMG-box. Bromodomains recognize and bind to acetylated lysines, the BAH (bromo-adjacent homology) domains mediate protein protein interactions, and the HMG-box domain binds to DNA. Nine isoforms of BAF180 have been described that are produced by alternative splicing. BAF180 serves as a subunit of the SWI/SNF complex and plays critical roles in the regulation of chromatin remodeling and gene transcription, and is reported to be frequently mutated in several human cancers. BAF180 acts asa negative regulator of cell proliferation and plays a role in suppression of tumorigenesis through its ability to regulate both baseline and signal-dependent p21 transcription by its binding of the p21 promoter. Mutations in PBRM1 gene are known to cause renal cell carcinoma. (Ref.: Lee, H., et al. (2016). Oncotarget 7(15);19134-46; Gao, W., et al. (2017). Proc. Natl. Acad. Sci. USA. 114(5):1027-1032).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-BAF180, clone 370.1.8C3, Cat. No. MABE1138, is a highly specific rat monoclonal antibody that detects Protein Polybromo-1 (BAF180) and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected BAF180 in Western Blotting applications (Gao, W., et. al. (2017). Proc Natl Acad Sci U S A. 114(5):1027-1032).
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 241 amino acids from the C-terminal half of human Protein polybromo-1 (BAF180).
Clone370.1.8C3
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 370.1.8C3 is a rat monoclonal antibody that specifically detects human Protein Polybromo-1 (BAF180). It targets an epitope within 241 amino acids from the C-terminal half.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PBRM1
  • BAF180
  • PB1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~210 kDa observed; 192.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected BAF180 in HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1138-25UL 04054839540271