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MABT95 Anti-Arp2/3 complex Antibody, clone 13C9

Anti-Arp2/3 complex Antibody, clone 13C9 detects level of Arp2/3 complex & has been published & validated for use in WB, IH(P).

Anti-Arp2/3 complex Antibody, clone 13C9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: ARPC3, ARC21 UniProt Number: O15145
MABT95
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT95
DescriptionAnti-Arp2/3 complex Antibody, clone 13C9
Alternate Names
  • Actin-related protein 2/3 complex subunit 3
  • Arp2/3 complex 21 kDa subunit
  • p21-ARC
Background InformationThe Arp2/3 complex, which contains ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC, plays a major role in the regulation of the actin polymerization in the cytoskeleton. Two of complex's seven subunits (ARP2 and ARP3) closely resemble monomeric actin and serve as nucleation sites for new actin filaments. The Arp2/3 complex works in conjunction with an activating nucleation-promoting factor (NPF) to aid in the formation of branched actin networks. The regulation of actin cytoskeleton reorganization is important for processes like cell migration, phagocytosis, and intracellular motility of lipid vesicles.
References
Product Information
FormatPurified
Control
  • MCF-7 cell lysate
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Arp2/3 complex Antibody, clone 13C9 detects level of Arp2/3 complex & has been published & validated for use in WB, IH(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:5,000 dilution from a representative lot detected Arp2/3 complex in human colorectal cancer and human colon tissues (antibody may need further dilution if staining is too strong - optimal dilution to be determined by the end user).
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human Arp2/3 complex.
Clone13C9
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p21 subunit, has yet to be determined. [provided by RefSeq, Jul 2008].
Gene Symbol
  • ARPC3
  • ARC21
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.

SUBUNIT STRUCTURE: Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.

SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Cell projection

SEQUENCE SIMILARITIES: Belongs to the ARPC3 family.
Molecular Weight~20 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: A 1:5,000 dilution of this antibody detected Arp2/3 complex on 10 µg of MCF-7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT95 04053252687778

Documentation

Anti-Arp2/3 complex Antibody, clone 13C9 SDS

Title

Safety Data Sheet (SDS) 

Anti-Arp2/3 complex Antibody, clone 13C9 Certificates of Analysis

TitleLot Number
Anti-Arp2/3 complex, clone 13C9 - 2388376 2388376
Anti-Arp2/3 complex, clone 13C9 - 2227684 2227684
Anti-Arp2/3 complex, clone 13C9 - 2283905 2283905
Anti-Arp2/3 complex, clone 13C9 - 2562396 2562396
Anti-Arp2/3 complex, clone 13C9 - 3173555 3173555
Anti-Arp2/3 complex, clone 13C9 - 3283230 3283230
Anti-Arp2/3 complex, clone 13C9 - 3432920 3432920
Anti-Arp2/3 complex, clone 13C9 - 3574550 3574550
Anti-Arp2/3 complex, clone 13C9 - 3827479 3827479
Anti-Arp2/3 complex, clone 13C9 - 4076695 4076695
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