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MAB348B Sigma-AldrichAnti-Alzheimer Precursor Protein A4 Antibody, clone 22C11, Biotin Conj.

Anti-Alzheimer Precursor Protein A4 Antibody, clone 22C11, Biotin Conj. detects level of Alzheimer Precursor Protein A4 & has been published & validated for use in IHC, ICC.

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Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: APP, A4, AD1 UniProt Number: P05067

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
R, M, Ca, H, Mk, Po	IHC, ICC	M	Biotin	Monoclonal Antibody

Description
Catalogue Number	MAB348B
Description	Anti-Alzheimer Precursor Protein A4 Antibody, clone 22C11, Biotin Conj.
Alternate Names	Amyloid beta A4 protein ABPP APPI APP Alzheimer disease amyloid protein Cerebral vascular amyloid peptide CVAP PreA4 Protease nexin-II PN-II N-APP Soluble APP-alpha S-APP-alpha Soluble APP-beta S-APP-beta C99 Beta-amyloid protein 42 Beta-APP42 Beta-amyloid protein 40 Beta-APP40 C83 P3(42) P3(40) C80 Gamma-secretase C-terminal fragment 59 Amyloid intracellular domain 59 AICD-59 AID(59) Gamma-CTF(59) Gamma-secretase C-terminal fragment 57 Amyloid intracellular domain 57 AICD-57 AID(57) Gamma-CTF(57) Gamma-secretase C-terminal fragment 50 Amyloid intracellular domain 50 AICD-50 AID(50) Gamma-CTF(50) C31
Background Information	Deposits of amyloid protein in senile plaques near nerve processes are found in the brains of aged humand and cases of Alzheimer's Disease. The principle component of this extracellular amyloid is beta A4, a 4 kDa peptide derived from a larger amyloid precursor protein (APP), which is widely expressed in the brain and body. The creation, transport and function of these proteins is currently an active area of research.

References

Product Information
Format	Biotin
Control	Rat E18 primary cortex cells
Presentation	Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality Level	MQ100

Applications
Application	Anti-Alzheimer Precursor Protein A4 Antibody, clone 22C11, Biotin Conj. detects level of Alzheimer Precursor Protein A4 & has been published & validated for use in IHC, ICC.
Key Applications	Immunohistochemistry Immunocytochemistry
Application Notes	Evaluated by Immunohistochemistry in adult rat brain tissue. Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Alzheimer Precursor Protein A4 in adult rat brain tissue.

Biological Information
Immunogen	Purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein
Epitope	N-terminus
Clone	22C11
Host	Mouse
Specificity	This antibody recognizes Alzheimer Precursor Protein A4 at the N-temrinus.
Isotype	IgG1
Species Reactivity	Rat Mouse Canine Human Monkey Pig
Species Reactivity Note	Demonstrated to react with Rat. Predicted to react with Mouse, Canine, Human, Monkey, and Porcine based on 100% sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_000475
Entrez Gene Summary	This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Gene Symbol	APP A4 AD1
Purification Method	Protein A Purfied
UniProt Number	P05067
UniProt Summary	FUNCTION: The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis. SIZE: 770 amino acids; 86943 Da SUBUNIT: Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, MAPK8IP1, SHC1 and, Numb and Dab1 (By similarity). Binding to Dab1 inhibits its serine phosphorylation (By similarity). Also interacts with GPCR-like protein BPP, FPRL1, APPBP1, IB1, KNS2 (via its TPR domains) (By similarity), APPBP2 (via BaSS) and DDB1. In vitro, it binds MAPT via the MT-binding domains (By similarity). Associates with microtubules in the presence of ATP and in a kinesin-dependent manner (By similarity). Interacts, through a C-terminal domain, with GNAO1. Amyloid beta-42 binds CHRNA7 in hippocampal neurons. Beta-amyloid associates with HADH2. Soluble APP binds, via its N-terminal head, to FBLN1. Interacts with CPEB1 (By similarity). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Note=Cell surface protein that rapidly becomes internalized via clathrin-coated pits. During maturation, the immature APP (N-glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O- glycosylated and sulfated). After alpha-secretase cleavage, soluble APP is released into the extracellular space and the C- terminal is internalized to endosomes and lysosomes. Some APP accumulates in secretory transport vesicles leaving the late Golgi compartment and returns to the cell surface. Gamma-CTF(59) peptide is located to both the cytoplasm and nuclei of neurons. It can be translocated to the nucleus through association with Fe65. Beta- APP42 associates with FRPL1 at the cell surface and the complex is then rapidly internalized. APP sorts to the basolateral surface in epithelial cells. During neuronal differentiation, the Thr-743 phosphorylated form is located mainly in growth cones, moderately in neurites and sparingly in the cell body. Casein kinase phosphorylation can occur either at the cell surface or within a post-Golgi compartment. TISSUE SPECIFICITY: Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Appican is expressed in astrocytes. DOMAIN: The basolateral sorting signal (BaSS) is required for sorting of membrane proteins to the basolateral surface of epithelial cells. & The NPXY sequence motif found in many tyrosine- phosphorylated proteins is required for the specific binding of the PID domain. However, additional amino acids either N- or C- terminal to the NPXY motif are often required for complete interaction. The PID domain-containing proteins which bind APP require the YENPTY motif for full interaction. These interactions are independent of phosphorylation on the terminal tyrosine residue. The NPXY site is also involved in clathrin-mediated endocytosis. PTM: Proteolytically processed under normal cellular conditions. Cleavage by alpha-secretase or alternatively by beta-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, respectively, and the retention of corresponding membrane-anchored C-terminal fragments, C83 and C99. Subsequent processing of C83 by gamma-secretase yields P3 peptides. This is the major secretory pathway and is non-amyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59). & Proteolytically cleaved by caspases during neuronal apoptosis. Cleavage at Asp-739 by either caspase-6, -8 or -9 results in the production of the neurotoxic C31 peptide and the increased production of beta-amyloid peptides. & N- and O-glycosylated. O-linkage of chondroitin sulfate to the L-APP isoforms produces the APP proteoglycan core proteins, the appicans. The chondroitin sulfate chain of appicans contains 4-O-sulfated galactose in the linkage region and chondroitin sulfate E in the repeated disaccharide region (By similarity). & Phosphorylation in the C-terminal on tyrosine, threonine and serine residues is neuron-specific. Phosphorylation can affect APP processing, neuronal differentiation and interaction with other proteins. Phosphorylated on Thr-743 in neuronal cells by Cdc5 kinase and Mapk10, in dividing cells by Cdc2 kinase in a cell- cycle dependent manner with maximal levels at the G2/M phase and, in vitro, by GSK-3-beta. The Thr-743 phosphorylated form causes a conformational change which reduces binding of Fe65 family members. Phosphorylation on Tyr-757 is required for SHC binding. Phosphorylated in the extracellular domain by casein kinases on both soluble and membrane-bound APP. This phosphorylation is inhibited by heparin. & Extracellular binding and reduction of copper, results in a corresponding oxidation of Cys-144 and Cys-158, and the formation of a disulfide bond. In vitro, the APP-Cu(+) complex in the presence of hydrogen peroxide results in an increased production of beta-amyloid-containing peptides. DISEASE: Defects in APP are a cause of autosomal dominant Alzheimer disease (AD) [MIM:104300]. AD is the most prevelant form of dementia, characterized by neurofibrillary tangles and amyloid plaques deposition in the brain. Identical lesions occur in the neurons of aged Down syndrome but at an earlier age than in AD. The major constituent of these neuritic plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. Mutations occurring at the beta-amyloid N-terminal, such as the Swedish double mutation, appear to increase levels of beta-amyloid by facilitating beta-secretase cleavage resulting in elevated levels of both beta-APP42 and beta-APP40. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31, are also implicated in AD neuronal death. Alzheimer disease caused by mutations in APP is a rare occurrence and usually causes the familial or early-onset form of the disease (FAD). Flemish-type AD is characterized by, in addition to presenile dementia, cerebral hemorrhaging due to cerebral amyloid angiopathy which is similar to, but distinct from, cerebroarterial amyloidosis Dutch type. Only about 5% of all cases of Alzheimer disease are caused by FAD mutations, the rest are sporadic. & Defects in APP are the cause of hereditary cerebral hemorrhage with amyloidosis Dutch type (HCHWAD) [MIM:609065]. HCHWAD is characterized by amyloid deposits in cerebral vessels. The principal clinical characteristics are recurring cerebral hemorrhages, sometimes preceded by migrainous headaches or mental cleavage. Beta-APP40 is the predominant form of cerebrovascular amyloid. & Defects in APP are the cause of hereditary cerebroarterial amyloidosis Iowa type [MIM:605714]. Hereditary cerebroarterial amyloidosis Iowa type is an autosomal dominant dementia beginning in the sixth or seventh decade of life. The patients have progressive aphasic dementia, leukoencephalopathy, and occipital calcifications. They do not present cerebral hemorrhaging. SIMILARITY: Belongs to the APP family. & Contains 1 BPTI/Kunitz inhibitor domain. MISCELLANEOUS: Chelation of metal ions, notably copper, iron and zinc, can induce histidine-bridging between beta-amyloid molecules resulting in beta-amyloid-metal aggregates. The affinity for copper is much higher than for other transient metals and is increased under acidic conditions. Extracellular zinc-binding increases binding of heparin to APP and inhibits collagen-binding.
Molecular Weight	Varies

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Product Usage Statements
Quality Assurance	Evaluated by Immunocytochemistry in rat E18 primary cortex cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Alzheimer Precursor Protein A4 in rat E18 primary cortex cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

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Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
MAB348B	04053252537233

Documentation

Anti-Alzheimer Precursor Protein A4 Antibody, clone 22C11, Biotin Conj. SDS

Title
Safety Data Sheet (SDS)

Anti-Alzheimer Precursor Protein A4 Antibody, clone 22C11, Biotin Conj. Certificates of Analysis

Title	Lot Number
Anti-Alzheimer Precursor Protein A4, -2806113	2806113
Anti-Alzheimer Precursor Protein A4, -2806130	2806130
Anti-Alzheimer Precursor Protein A4, clone 22C11, Biotin Conj.	3075003
Anti-Alzheimer Precursor Protein A4, clone 22C11, Biotin Conj. - 2397169	2397169
Anti-Alzheimer Precursor Protein A4, clone 22C11, Biotin Conj. - 2106931	2106931
Anti-Alzheimer Precursor Protein A4, clone 22C11, Biotin Conj. - 2196095	2196095
Anti-Alzheimer Precursor Protein A4, clone 22C11, Biotin Conj. - 2207236	2207236
Anti-Alzheimer Precursor Protein A4, clone 22C11, Biotin Conj. - 2275642	2275642
Anti-Alzheimer Precursor Protein A4, clone 22C11, Biotin Conj. - 2561141	2561141
Anti-Alzheimer Precursor Protein A4, clone 22C11, Biotin Conj. - 3160188	3160188

Newsletters / Publications

Title
Research Focus - Volume 2, 2013

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Life Science Research > Antibodies and Assays > Primary Antibodies

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