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MABF2087-100UL Anti-Adenylate Cyclase Toxin Antibody, B. pertussis Antibody, clone 9D4

Anti-Adenylate Cyclase Toxin, B. pertussis, clone 9D4, Cat. No. MABF2087 is a mouse monoclonal antibody that detects Adenylate cyclase (AC) toxin from Bordetella pertussis and has been tested for use in Dot Blot, Function Assay and Western Blotting.

MABF2087-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2087-100UL
DescriptionAnti-Adenylate Cyclase Toxin Antibody, B. pertussis Antibody, clone 9D4
Alternate Names
  • Adenylate cyclase toxin
  • Bordetella pertussis Toxin
  • ACT
Background InformationAdenylate Cyclase Toxin (UniProt: C8C508; also known as ACT or Cya A) is one of several virulence factors produced by the bacterium Bordetella pertussis. Bordetella pertussis, a gram-negative bacterium, is shown to be the causative agent for whooping cough. It produces several virulence factors, including Cya A. The CyaA toxin is a bifunctional protein of 1,706 residues consisting of an NH2-terminal catalytic domain of 400 amino acids and a COOH-terminal part with 1,306 residues. The CyaA polypeptide is synthesized as an inactive protoxin, which is then converted to an active toxin by posttranslational palmitoylation of two internal lysine residues (Lys 856 and 963). Upon interaction of its C-terminal hemolysin moiety with the cell surface receptor alphaM-beta2 integrin ((Cd11b/Cd18), the N-terminal cyclase domain translocates into the host cell cytosol where it generates supraphysiological levels of cAMP concentration, which inhibits host cell anti-bacterial activities and apoptotic death of macrophages. Cya A toxin is also shown to elicit potassium ion efflux from erythrocytes in a process that is thought to be a precursor event to osmotic lysis of erythrocytes. Clone 9D4 binds to an epitope of AC toxin in the glycine- and aspartate- rich region. Hence, it recognizes other RTX toxins in addition to AC toxin. Clone 9D4 increases the fraction of AC toxin (CyaA) that is secreted to cell surface and release of CyaA into culture supernatant(Ref.: Guermonprez, P., et al. (2001). J. Exp. Med. 193(9); 1035-1044; Gray, MC., et al. (2001). J. Bacteriol. 183(20); 5904-5910; Holubova, J., et al. (2012). Infect. Immun. 80(3); 1181-1192).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in PBS without preservatives.
Applications
ApplicationAnti-Adenylate Cyclase Toxin, B. pertussis, clone 9D4, Cat. No. MABF2087 is a mouse monoclonal antibody that detects Adenylate cyclase (AC) toxin from Bordetella pertussis and has been tested for use in Dot Blot, Function Assay and Western Blotting.
Key Applications
  • Dot Blot
  • Function Assay
  • Western Blotting
Application NotesFunction Analysis: A representative lot increased the fraction of AC toxin (CyaA) that is secreted to cell surface and release of CyaA into culture supernatant. (Gray, M.C., et. al. (2001). J Bacteriol. 183(20):5904-10).

Western Blotting Analysis: A representative lot detected Adenylate Cyclase Toxin, B. pertussis in Western Blotting applications (Holubova, J., et. al. (2012). Infect Immun. 80(3):1181-92; Lee, S.J., et. al. (1999). Infect Immun. 67(5):2090-5).

Dot Blot Analysis: A representative lot detected Adenylate Cyclase Toxin, B. pertussis in Dot Blot applications (Lee, S.J., et. al. (1999). Infect Immun. 67(5):2090-5).
Biological Information
ImmunogenAdenylate cyclase toxin from Bordetella pertussis.
Clone9D4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 9D4 is a mouse monoclonal antibody that detects Adenylate cyclase (AC) toxin from Bordetella pertussis.
IsotypeIgG2aλ
Species Reactivity
  • Bacteria
Species Reactivity NoteBacteria.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
Molecular Weight~220 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Adenylate cyclase toxin from Bordetella pertussis.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Adenylate Cyclase Toxin from from Bordetella pertussis.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2087-100UL 04054839499593

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