Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN2266 Anti-ARID1B Antibody, clone 3D9.1

Anti-ARID1B, clone 3D9.1, Cat. No. MABN2266, is a highly specific mouse monoclonal antibody that targets AT-rich interactive domain-containing protein 1B and has been tested for use in Immunocytochemistry and Western Blotting.

Anti-ARID1B Antibody, clone 3D9.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: ARID1B, BAF250B, DAN15, KIAA1235, OSA2 UniProt Number: Q8NFD5
MABN2266
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2266
DescriptionAnti-ARID1B Antibody, clone 3D9.1
Alternate Names
  • AT-rich interactive domain-containing protein 1B
  • ARID domain-containing protein 1B
  • BRG1-associated factor 250b
  • BAF250B
  • BRG1-binding protein hELD/OSA1
  • Osa homolog 2
  • hOsa2
  • p250R
Background InformationAT-rich interactive domain-containing protein 1B (UniProt: Q8NFD5; also known as ARID domain-containing protein 1B, BRG1-associated factor 250b, BAF250B, BRG1-binding protein hELD/OSA1, Osa homolog 2, hOsa2, p250R) is encoded by the ARID1B (also known as BAF250B, DAN15, KIAA1235, OSA2) gene (Gene ID: 57492) in human. ARID domain-containing protein 1B (ARID1B) is widely expressed with high levels in heart, skeletal muscle, and kidney. Four isoform of ARID1B have been reported that are produced by alternative splicing. Its ARID domain is localized to amino acids 1053-1144. ARID1B is a component of the SWI/SNF chromatin remodeling complex and may play a role in cell-cycle activation. It is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). ARID1B and ARID1A display about 60% homology, but have been reported to have opposing functions in cell cycle arrest and are mutually exclusive since individual SWI/SNF chromatin remodeling complexes can contain either ARID1A or ARID1B, but not both. ARID1B belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. Mutations in ARID1B gene are linked to the development of Coffin-Siris syndrome 1, a congenital multiple malformation syndrome with intellectual disability, coarse facial features, and malformations of the cardiac, gastrointestinal, genitourinary, and/or central nervous systems.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-ARID1B, clone 3D9.1, Cat. No. MABN2266, is a highly specific mouse monoclonal antibody that targets AT-rich interactive domain-containing protein 1B and has been tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:250 dilution from a representative lot detected ARID1B in HeLa, A431, HUVEC and NIH/3T3 cells.
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 138 amino acids from the N-terminal half of human AT-rich interactive domain-containing protein 1B.
Clone3D9.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3D9.1 detects AT-rich interactive domain-containing protein 1B (ARID1B) in human and murine cells. It targets an epitope within 138 amino acids from the N-terminal half.
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ARID1B
  • BAF250B
  • DAN15
  • KIAA1235
  • OSA2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~300 kDa observed; 236.12 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ARID1B in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2266 04054839412370