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MABN2297 Anti-APP Antibody, clone 7H6

Anti-APP, clone 7H6, Cat. No. MABN2297, is a highly specific rat monoclonal antibody, that targets Amyloid beta A4 protein and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Anti-APP Antibody, clone 7H6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: APP, A4, AD1 UniProt Number: P05067
MABN2297
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, ICC, IP, WB, FCRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2297
DescriptionAnti-APP Antibody, clone 7H6
Alternate Names
  • Amyloid beta A4 protein
  • ABPP
  • APPI
  • Alzheimer disease amyloid protein
  • Amyloid precursor protein
  • Beta-amyloid precursor protein
  • Cerebral vascular amyloid peptide
  • CVAP
  • PreA4
  • Protease nexin-II
  • PN-II
Background InformationAmyloid beta A4 protein (UniProt: P05067; also known as ABPP, APPI, APP, Alzheimer disease amyloid protein, Amyloid precursor protein, Beta-amyloid precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID: 351) in human. APP undergoes extensive post-translational modification including glycosylation, phosphorylation, and tyrosine sulfation, as well as many types of proteolytic processing to generate peptide fragments. APP is proteolytically processed under normal cellular conditions by alpha-secretase or beta-secretase to generate and release soluble APP peptides, S-APP-alpha and S-APP-beta, and the retention of corresponding membrane-anchored C-terminal fragments, C80, C83 and C99. Subsequent processing of C80 and C83 by gamma-secretase yields P3 peptides. In Alzheimer s disease processing of C99 generates amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42) that form amyloid plaques. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. They bind transient metals such as copper, zinc and iron. APP can also be cleaved by caspases during neuronal apoptosis. Cleavage at Asp-739 by either caspase-6, -8 or -9 results in the production of the neurotoxic C31 peptide and the increased production of beta-amyloid peptides. In addition to its obvious role in Alzheimer's disease, the most-substantiated role for APP is in synaptic formation and repair. Its expression is upregulated during neuronal differentiation and after neural injury.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-APP, clone 7H6, Cat. No. MABN2297, is a highly specific rat monoclonal antibody, that targets Amyloid beta A4 protein and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • ELISA
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blotting
  • Flow Cytometry
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected APP in HEK293 cells.

Western Blotting Analysis: A representative lot detected APP in Western Blotting applications (Hoefling, C., et. al. (2016). Aging Cell. 15(5):953-63).

Flow Cytometry Analysis: A representative lot detected APP in Flow Cytometry applications (Hoefling, C., et. al. (2016). Aging Cell. 15(5):953-63).

Western Blotting Analysis: A 1:10 dilution from a representative lot detected App in WT and APP knockdown HEK293T cells (Courtesy of Dr. med. Peer-Hendrik Kuhn, Ph.D., Institut fur Allgemeine Pathologie und Pathologische Anatomie, Technische Universität München, Munich, Germany).

Immunoprecipitation Analysis: A representative lot detected APP in Immunoprecipitation applications (Hoefling, C., et. al. (2016). Aging Cell. 15(5):953-63).

ELISA Analysis: A representative lot detected APP in ELISA applications (Hoefling, C., et. al. (2016). Aging Cell. 15(5):953-63).

Immunocytochemistry Analysis: A 1:10 dilution from a representative lot detected APP in HEK293T either lentivirally transduced with APP shRNA-1 or APP shRNA-2 both coexpressing GFP as a reporter (Courtesy of Dr. med. Peer-Hendrik Kuhn, Ph.D., Institut fur Allgemeine Pathologie und Pathologische Anatomie, Technische Universität München, Munich, Germany).

Immunocytochemistry Analysis: A representative lot detected APP in Immunocytochemistry applications (Hoefling, C., et. al. (2016). Aging Cell. 15(5):953-63).
Biological Information
ImmunogenStrepII-tagged human recombinant ectodomain region of the neuronal isoform hAPP695 lacking the KPI domain.
Clone7H6
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 7H6 specifically detects human amyloid precursor protein (APP) and does not display reactivity with other species. It targets an epitope within the ectodomain region of APP.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • APP
  • A4
  • AD1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~95 kDa observed; 86.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected APP in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2297 04054839415289