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MABN2637-25UG Anti-AIDA1 Antibody, clone 2B22

Anti-AIDA1, clone 2B22, Cat. No. MABN2637, is a mouse monoclonal antibody that detects AIDA1/ANKS1B and is tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

MABN2637-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABN2637-25UG
DescriptionAnti-AIDA1 Antibody, clone 2B22
Alternate Names
  • Alt Names: Ankyrin repeat and sterile alpha motif domain-containing protein 1B
  • Amyloid-beta protein intracellular domain-associated protein 1
  • ANKS1B
  • E2A-PBX1-associated protein
  • EB-1
Background InformationAnkyrin repeat and sterile alpha motif domain-containing protein 1B (UniProt: P0C6S7; also known as Amyloid-beta protein intracellular domain-associated protein 1, AIDA-1, E2A-PBX1-associated protein, EB-1) is encoded by the Anks1b gene (Gene ID: 314721) in rat. AIDA-1 is an adaptor protein that has been implicated in Cajal body and nucleolar function. It is expressed throughout the brain and is highly expressed in the hippocampus. It is one of the most abundant proteins at postsynaptic densities where it is reported to bind to PSD95 and associates with NMDA receptors (NMDARs). In the brain it interacts with amyloid b precursor protein and plays a role in brain development. AIDA-1 preferentially associates with GluN2B-containing NMDARs and plays a role in their transport from the endoplasmic reticulum (ER). Loss of AIDA-1 results in the accumulation of GluN2B in the ER and decreases GluN2B and increases GluN2A distribution at the synapse. However, loss of AIDA-1 does not affect AMPAR-mediated synaptic transmission or the probability of glutamate release. Mutations in Anks1b gene are known to affect GluN2B function and are linked to the pathogenesis of neuropsychiatric disorders. (Ref.: Carbonell, AU., et al., (2019). Nat. Commun. 10(1); 3529; Tindi, JO., et al. (2015). J. Neurosci. 35(24); 8986- 8996).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-AIDA1, clone 2B22, Cat. No. MABN2637, is a mouse monoclonal antibody that detects AIDA1/ANKS1B and is tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesTested Applications

Western Blotting Analysis: A representative lot detected AIDA1 in Western Blotting applications (Tindi, J.O., et al. (2015). J Neurosci. 35(24): 8986-96).

Immunofluorescence Analysis: A representative lot detected AIDA1 in Immunofluorescence applications (Tindi, J.O., et al. (2015). J Neurosci. 35(24): 8986-96).

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected AIDA1 in rat cerebral cortex, hippocampus, and cerebellum tissue sections.

Immunoprecipitation Analysis: A representative lot immunoprecipitated AIDA1 in Immunoprecipitation applications (Carbonell, A.U., et al. (2019). Nat Commun.10(1): 3529).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenA linear peptide corresponding to 10 amino acids from the C-terminal region of rat Ankyrin repeat and sterile alpha motif domain-containing protein 1B (AIDA-1).
EpitopeC-terminal
Clone2B22
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 2B22 is a is a mouse monoclonal antibody that detects AIDA-1. It targets an epitope within 10 amino acids from the C-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Rat
Species Reactivity NoteRat. Predicted to react with Canine, Feline based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Anks1b
Purification MethodProtein G purified
UniProt Number
Molecular Weight~50 and 55 kDa observed; 139.21, 48.37, and 57.81 kDa, respectively for isoforms 1, 2, and 3. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AIDA1 in Rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2637-25UG 04065267027791