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121800 Advanced Glycation Endproduct-BSA

AGE-BSA has been reported to induce apoptosis in cultured human umbilical vein endothelial cells and inhibit nitric oxide synthase activity in proximal tubular epithelial cells.

Advanced Glycation Endproduct-BSA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: AGE-BSA

121800
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
121800-10MG Glass bottle 10 mg
Description
OverviewPrepared by reacting BSA with glycoaldehyde under sterile conditions. Glycated-BSA shows a 5,000 - 10,000% increase in fluorescence as compared to normal BSA (confirmed by fluorescence spectrophotometry, excitation/emission 370/440 nm). AGE-BSA has been reported to induce apoptosis in cultured human umbilical vein endothelial cells and inhibit nitric oxide synthase activity in proximal tubular epithelial cells. Advanced glycation end products and their receptors have been implicated in the pathogenesis of diabetes, induction of proinflammatory cytokines, and stimulation of smooth muscle proliferation, and fibronectin production.
Catalogue Number121800
Brand Family Calbiochem®
SynonymsAGE-BSA
References
ReferencesOkamoto, T., et al. 2002. Microvasc. Res. 63, 186.
Ohgami, N., et al. 2001. J. Biol. Chem. 276, 3195.
Wang, R., et al. 2001. J. Nippon Med. Sch. 68, 472.
Sakata, N., et al. 2000. J. Atheroscler. Thromb. 7, 169.
Verbeke, P., et al. 2000. Biochim. Biophys. Acta 1502, 481.
Farboud, B., et al. 1999. Mol. Vis. 5, 11.
Huang, J.-S., et al. 1999. Biochem. J. 342, 231.
Min, C., et al. 1999. Diabetes Res. Clin. Pract. 46, 197.
Neumann, A., et al. 1999. FEBS Lett. 453, 283.
Stitt, A.W., et al. 1999. Biochem. Biophys. Res. Commun. 256, 549.
Nazaimoon, W. and Bak, K. 1998. Malays. J. Pathol. 20, 83.
Product Information
FormLiquid
FormulationIn sterile-filtered PBS.
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
121800-10MG 04055977206197

Documentation

Advanced Glycation Endproduct-BSA Certificates of Analysis

TitleLot Number
121800

References

Reference overview
Okamoto, T., et al. 2002. Microvasc. Res. 63, 186.
Ohgami, N., et al. 2001. J. Biol. Chem. 276, 3195.
Wang, R., et al. 2001. J. Nippon Med. Sch. 68, 472.
Sakata, N., et al. 2000. J. Atheroscler. Thromb. 7, 169.
Verbeke, P., et al. 2000. Biochim. Biophys. Acta 1502, 481.
Farboud, B., et al. 1999. Mol. Vis. 5, 11.
Huang, J.-S., et al. 1999. Biochem. J. 342, 231.
Min, C., et al. 1999. Diabetes Res. Clin. Pract. 46, 197.
Neumann, A., et al. 1999. FEBS Lett. 453, 283.
Stitt, A.W., et al. 1999. Biochem. Biophys. Res. Commun. 256, 549.
Nazaimoon, W. and Bak, K. 1998. Malays. J. Pathol. 20, 83.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Pathways and Biomarkers of Oxidative Stress
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-April-2008 RFH
SynonymsAGE-BSA
DescriptionPrepared by reacting bovine serum albumin (BSA) with glycoaldehyde under sterile conditions. Fluorescence of AGE-BSA is confirmed by fluorescence spectrophotometry, excitation/emission = 370/440 nm. Glycated BSA shows a 5000-10,000% increase in fluorescence compared to control BSA. AGE and their receptors have been implicated in the pathogenesis of diabetes, induction of pro-inflammatory cytokines, and stimulation of smooth muscle proliferation and fibronectin production. AGE-BSA has also been shown to induce apoptosis in cultured human umbilical vein endothelial cells (HUVEC) and inhibit nitric oxide synthase activity in proximal tubular epithelial cells of the kidney.
FormLiquid
FormulationIn sterile-filtered PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by SDS-PAGE
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesOkamoto, T., et al. 2002. Microvasc. Res. 63, 186.
Ohgami, N., et al. 2001. J. Biol. Chem. 276, 3195.
Wang, R., et al. 2001. J. Nippon Med. Sch. 68, 472.
Sakata, N., et al. 2000. J. Atheroscler. Thromb. 7, 169.
Verbeke, P., et al. 2000. Biochim. Biophys. Acta 1502, 481.
Farboud, B., et al. 1999. Mol. Vis. 5, 11.
Huang, J.-S., et al. 1999. Biochem. J. 342, 231.
Min, C., et al. 1999. Diabetes Res. Clin. Pract. 46, 197.
Neumann, A., et al. 1999. FEBS Lett. 453, 283.
Stitt, A.W., et al. 1999. Biochem. Biophys. Res. Commun. 256, 549.
Nazaimoon, W. and Bak, K. 1998. Malays. J. Pathol. 20, 83.