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116450 AFB-Color staining kit

for the microscopic investigation of acid-fast bacteria (cold staining)

AFB-Color staining kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
116450
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1164500001 Fibre carton 1 set
Description
Catalogue Number116450
DescriptionAFB-Color staining kit
OverviewThe AFB-Color staining kit - for the microscopic investigation of acid-fast bacteria (AFB) (cold staining), is a ready-to-use solution used for human-medical cell diagnosis and serves the purpose of the bacteriological investigation of sample material of human origin, for example smears of enriched bacterial cultures. The AFB-Color staining set is based on the Ziehl-Neelsen staining method for the detection of acid-fast bacteria. The set is modified such, that heating of the carbolfuchsin solution is dispensable. Consequently, the release of potentially hazardous phenolic vapors will be avoided.The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus the formation of the mycolate-fuchsin complex in the cell wall. The AFB-Color Kit offers a modified AFB-Color Carbol fuchsin solution which abolishes heating (Kinyoun method, cold staining).Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye.
Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. The package is sufficient for up to 250 applications.This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Applicationfor the microscopic investigation of acid-fast bacteria (cold staining)
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H225: Highly flammable liquid and vapour.
H290: May be corrosive to metals.
H314: Causes severe skin burns and eye damage.
H341: Suspected of causing genetic defects.
H410: Very toxic to aquatic life with long lasting effects.
EUH031: Contact with acids liberates toxic gas.
Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P273: Avoid release to the environment.
P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
P304 + P340 + P310: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal WordDanger
Storage class3 Flammable liquids
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 3316 Entzündbarer flüssiger Stoff, giftig, n.a.g.(PHENOL UND ENTH. ETHANOL), 9, II
Declaration (transport by air) IATA-DGRUN 3316 FLAMMABLE LIQUID, TOXIC, N.O.S.(PHENOL AND CONT. ETHANOL), 9, II
Declaration (transport by sea) IMDG-CodeUN 3316 FLAMMABLE LIQUID, TOXIC, N.O.S.(PHENOL AND CONT. ETHANOL), 9, II
Supplemental Information
Specifications
Suitability for microscopypasses test
Mycobacteriared
Backgroundgreen
Global Trade Item Number
Catalogue Number GTIN
1164500001 04022536165912

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Catalogue Number Description
100327 Hydrochloric acid in ethanol
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109016 Neo-Mount™
109215 Ziehl-Neelsen carbol-fuchsin solution

Categories

IVD/OEM Materials & Reagents > Microscopy > Histology > Histological Staining Kits
IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Acid-Fast Bacteria Staining