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SCC504 3dGRO™ Human CRC Organoids (ISO50)

Ready-to-Assay human colorectal cancer organoids for 3D cell culture applications.

SCC504
≥ 100,000 organoids/vial  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCC504
Description3dGRO™ Human CRC Organoids (ISO50)
OverviewColorectal cancer accounts for roughly 10% of all cancer cases worldwide with more than half of all patients with CRC developing metastatic disease leading to death. Recently, tissue derived organoids have emerged as a more predictive 3 dimensional cell culture model of disease. 3D organoid cultures conserve the original genetic and phenotypic characteristics of the primary tissue allowing for their application in many research fields included drug development, personalized medicine and potential therapeutics. In vitro cultured tumor organoids have also been shown to predict patient response to chemotherapeutics. PDOs derived from colorectal cancer (CRC organoids) have been used for cell modeling and to investigate the function of cancer related driver gene mutations including APC, TP53, KRAS, BRAF, PIK3CA etc.

3dGRO™ Human CRC Organoids are offered in a ready-to-assay format intended to simplify the organoid cell culture workflow. A single vial of organoids is enough to directly thaw and seed into a 96-well plate without cell expansion for drug screening applications. The organoids are manufactured using Cellesce’s patented technology which enables the robust growth and expansion of patient-derived organoids (PDOs). Cellesce’s technology minimizes manual handling time to maximize reproducibility in order to position organoid cell models as a cost effective and accurate tool in early-stage drug discovery. The organoid biobank includes tumor derived colorectal cancer PDOs from a range of genetic backgrounds, driver gene mutations, tumor sites and cancer stages. These organoid cell lines have been well characterized and are all validated for response against a number of known CRC-targeting agents.

LM Badder, et al. 3D imaging of colorectal cancer organoids identifies responses to Tankyrase inhibitors. PLoS One. 2020 Aug 18;15(8):e0235319.
Alternate Names
  • Colorectal Cancer Organoid
  • Colon Tumor Organoid
  • Colon Organoid
References
Product Information
Components
  • 1 vial of ≥ 100,000 organoids
Applications
ApplicationReady-to-Assay human colorectal cancer organoids for 3D cell culture applications.
Key Applications
  • Stem Cell Culture
  • Cell Culture
Biological Information
HostHuman
Cell Line Type
  • Cancer Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceCell Growth: Organoid expansion after 7 days
Mycoplasma: Negative
Viral Testing: Negative (HIV-1, HIV-2, HBV, CMV, EBV, HPV)
Sterility (Bacteria, Yeast, Fungi): Negative
Mutational Profile: APC ∆R232*E1286*, TP53 ∆R248Q", KRAS ∆G12D, SMAD4 ∆E526K, SMAD2 ∆S464*
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore 3dGRO™ Human CRC Organoids at -80°C. For long term storage the cells should be stored in liquid nitrogen.
Packaging Information
Material Size≥ 100,000 organoids/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCC504 04065266299144