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SCC321 3dGRO™ Colon Intestinal Organoids, Age 21 (Prep 87-C)

Cryopreserved patient derived gastrointestinal organoids (PDOs)

SCC321
≥1500 viable organoids/vial  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberSCC321
Description3dGRO™ Colon Intestinal Organoids, Age 21 (Prep 87-C)
Alternate Names
  • Gut Organoids
  • Human PDO Biobank
Background InformationPatient derived organoids (PDOs) are novel in vitro 3D cell models that preserve original tissue physiology and molecular pathology, thus representing a clinically-relevant alternative to traditional 2D cell lines and an effective tool to refine and reduce animal models. PDOs can be derived from adult patient biopsies or resected tissues containing native LGR5+ stem cell populations and cultured within an ECM-rich substrate using specialized organoid media such as L-WRN conditioned media. Adult tissue derived organoids are phenotypically and genetically stable in long term culture, presenting more mature phenotypes compared to iPSC-derived organoids. Importantly, PDOs have shown to be able to predict patient clinical responses to chemotherapeutics.

We are now offering a comprehensive biobank of highly characterized tissue-derived human gastrointestinal organoids from normal and diseased patients. The intestinal organoid biobank contains over 50 highly characterized intestinal organoids from both normal and diseased patients derived from multiple regions of the digestive systems including small intestine (duodenum, ileum), stomach, rectum and colon.

Cell Line Characteristics
• Sex: Male
• Age: 21 Years
• Organ: Colon
• Disease: Normal
Applications
ApplicationCryopreserved patient derived gastrointestinal organoids (PDOs)
Key Applications
  • Stem Cell Culture
Application Notes3dGRO™ is a trademark of Merck KGaA, Darmstadt, Germany.
3dGRO™ organoids were derived utilizing HUB Organoid Technology.
The purchaser of this product shall agree to HUB’s terms of use, which shall be separately acknowledged and accepted by such purchaser, prior to transfer of this product to purchaser.
Biological Information
Cell Line Type
  • Organoid
Product Usage Statements
Quality Assurance• Viability: ≥1500 viable organoids/vial
• Organoid Growth: Pass
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Mycoplasma Contamination: Negative
• STR Profile: Pass
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore in liquid nitrogen. The organoids can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size≥1500 viable organoids/vial
Global Trade Item Number
Catalogue Number GTIN
SCC321 04061842199211