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565758 β-Secretase Substrate IV, Fluorogenic - CAS 310427-94-2 - Calbiochem

β-Secretase Substrate IV, Fluorogenic, is a highly sensitive FRET substrate. Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: FS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic

565758
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Overview

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Catalogue NumberPackaging Qty/Pack
565758-1MG Plastic ampoule 1 mg
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Description
OverviewA highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP). Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement. kcat/Km values (min-1/µM) at 37°C, pH 4.5, for pro-memapsin 2 and its activated species Leu28p-memapsin 2 are 0.044 and 0.040, respectively.
Catalogue Number565758
Brand Family Calbiochem®
SynonymsFS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic
References
ReferencesErmolieff, J., et al. 2000. Biochemistry 39, 12450.
Product Information
CAS number310427-94-2
ATP CompetitiveN
FormRed lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₉₁H₁₂₉N₂₅O₂₅S
Chemical formulaC₉₁H₁₂₉N₂₅O₂₅S
ReversibleN
Quality LevelMQ100
Applications
Applicationβ-Secretase Substrate IV, Fluorogenic, is a highly sensitive FRET substrate. Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement.
Biological Information
Primary Targetβ-secretase substrate
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceH-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Global Trade Item Number
Catalogue Number GTIN
565758-1MG 04055977267709