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K Values in Olive Oil

Special Application Package for Merck Spectrophotometers


Spectroquant® Prove Spectrophotometer

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The Green Oil with Golden Benefits

Olive oil is an edible fat derived from grinding ripe olives and extracting the oil. It is associated with numerous health benefits, and is said to protect from coronary heart disease, cancer, high blood pressure, and other illnesses. Besides its significant role in nutrition, olive oil is also used in cosmetics, and pharmaceuticals as well as for industrial purposes.Spectroquant® Pharo Spectrophotometers

Originally from the Mediterranean region, olive oil is produced and consumed throughout the world today. There are various quality grades available from extra virgin to residue olive oil.

Measuring Oxidation in Olive Oil

Oxidation of olive oil causes the formation of conjugated diene and triene systems, which absorb ultraviolet light at wavelengths of 232 to 272 nm. In the EEC method, olive oil is dissolved in a specified solvent, and absorption is measured at wavelengths of 232, 264, 268, and 272 nm. Higher absorption at these wavelengths indicates a greater degree of oxidation and lower quality. Generally, the lower the Delta K value is, the purer and fresher the oil.

The European Quality Standard

To ensure the safety and accurate classification of olive oil, the European Commission introduced a standardized method for quality control. Commission Regulation (EEC) No 2568/91 relates to the characteristics of olive oil and olive-residue oil and the relevant methods of analysis. Annex IX of the Regulation describes a method for the analysis of olive oil via ultraviolet spectrometry, which can provide information on its quality, its state of preservation, and changes caused by production processes.

Producers’ Challenge: Satisfying Regulators & Consumers
  • Quality control analyses are essential for producers, not only to comply with regulations, but also to assess the quality, freshness and shelf life of their oils, and market them for a suitable price to consumers. 
  • However, the analysis and calculations require considerable time and effort.
No Calibrations or Calculations Needed
  • Pre-programmed for the Spectroquant® spectrophotometers, the K value applications allow you to accurately measure the specific extinction of olive oila at different wavelengths according to the requirements of Regulation (EEC) No 2568/91 Annex IX.
  • You won’t need to perform any calibrations or calculations. The photometer is pre-calibrated and calculates the K values automatically after measuring the absorption. 
  • Interested in fast, easy and error-free analysis? Request your copy of the application by contacting Merck.

Application Content

  1. Specific extinction (K232 nm value) of olive oil corresponding to Commission Regulation (EEC) No 2568/91 Annex IX
    Criterion for the durability of olive oil.
  2. Specific extinction (K270 nm value) of olive oil corresponding to Commission Regulation (EEC) No 2568/91 Annex IX
    Criterion for the freshness of olive oil (cyclohexane as solvent).
  3. Specific extinction (K268 nm value) of olive oil corresponding to Commission Regulation (EEC) No 2568/91 Annex IX
    Equivalent to K270, however, if isooctane is used as solvent, absorption must be measured at 268 nm.
  4. Specific extinction (ΔK270nm value) of olive oil corresponding to Commission Regulation (EEC) No 2568/91 Annex IX
    Criterion for pureness and quality of olive oil. ΔK270 value calculated using an equation based on K270 and K232 values.
  5. Specific extinction (ΔK268nm value) of olive oil corresponding to Commission Regulation (EEC) No 2568/91 Annex IX
    Equivalent to ΔK270, but using the K268 value.

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