71576 Sigma-AldrichpTriEx™-6 3C/LIC Vector Kit - Novagen
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Overview
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| Description | |
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| Overview | The pTriEx™-6 3C/LIC vector is a 3C/LIC
version of pTriEx-6. The pTriEx-6 3C/LIC
vector is uniquely designed to allow rapid
characterization of target genes in multiple
expression systems. With this vector, a single
recombinant plasmid can be used to test
expression in E. coli, insect, and mammalian
cells. Transient mammalian expression
is mediated by the CMV immediate early
enhancer and promoter. For expression
in insect cells, the pTriEx-6 3C/LIC vector
contains flanking baculovirus sequences
to permit the generation of recombinant
baculoviruses using the BacVector® System or
BacMagic™ Kits. In baculovirus-infected insect
cells, expression is driven by the very late p10
promoter. Expression in E. coli is regulated
by the tightly controlled T7lac promoter.
The pTriEx-6 3C/LIC vector contains an
N-terminal 8 aa Strep•Tag® II coding sequence
followed by a HRV 3C protease cleavage site
and a multiple cloning site, with the option
to encode optional sequences for a C-terminal
thrombin cleavage site and a His•Tag®
(10-histidine) fusion.
* Strep•Tag® technology for protein purification and detection is covered under US Patent No. 5,506,121, UK Patent No. 2,272,698 and French Patent No. 93 13 066. Strep•Tactin® is covered by US Patent No. 6,103,493. These products are provided only for research use. Information about licenses for commercial use is available from IBA GmbH, Rudolf-Wissell-Str. 28, D-37079 Goettingen, Germany. The pTriEx vector is covered by US Patent 6,589,783 owned by EMD Millipore Corporation or its Affiliates. This Product is sold for research use only. Any commercial use of the Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Further, the pTriEx vector allows for expression of target genes under control of the T7 promoter and is also covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs for research use only. Commercial entities need to obtain a research use license from Brookhaven National Labs prior to use of this Product. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product. |
| This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. | |
| Catalogue Number | 71576 |
| Brand Family | Novagen® |
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| Product Information | ||
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| • | 1 µg | pTriEx-6 3C/LIC Vector |
| • | 8 µl | 3C/LIC β-gal Control Insert |
| • | 25 U | T4 DNA Polymerase (LIC-qualified) |
| • | 50 µl | 10X T4 DNA Polymerase Buffer |
| • | 100 µl | 100 mM DTT |
| • | 40 µl | 25 mM dATP |
| • | 50 µl | 25 mM EDTA |
| • | 1.5 ml | Nuclease-free Water |
| • | 22 × 50 µl | NovaBlue GigaSingles™ Competent Cells |
| • | 2 × 0.2 ml | Origami™ B(DE3)pLacI Competent Cells |
| • | 5 × 2 ml | SOC Medium |
| • | 10 µl | Test Plasmid (ampicillin resistant) |
| Quality Level | MQ100 | |
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| Product Usage Statements |
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| Storage and Shipping Information | |
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| Ship Code | Dry Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | ≤ -70°C |
| Do not freeze | Ok to freeze |
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| Supplemental Information |
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| Specifications |
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| Global Trade Item Number | |
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| Catalogue Number | GTIN |
| 71576 | 0 |
Documentation
pTriEx™-6 3C/LIC Vector Kit - Novagen Certificates of Analysis
| Title | Lot Number |
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| 71576 |
User Protocols
| Title |
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| TB163 Ek/LIC Cloning Kits |
| TB250 pTriEx™ System Manual |
| TB453 3C/LIC Cloning Kits |
Vector Map
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| TB443VM pTriEx-6 3C/LIC Vector Map |
Vector Sequence
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| pTriEx-6 3C/LIC Vector Sequence |