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71237 pIEx™-1 Ek/LIC Vector Kit

71237
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      The pIEx™-1 Ek/LIC Vector is designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. This vector features the hr5 enhancer and the IE1 (immediate early) promoter to direct expression in insect cells. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and the associated cytotoxic effects. Cloning sites are available for producing fusion proteins also containing cleavable His•Tag® and S•Tag™ sequences for detection, purification and quantification. A C-terminal HSV•Tag® sequence serves as a cloning option to permit detection of target protein with the HSV•Tag Monoclonal Antibody.

      Catalogue Number71237
      Brand Family Novagen®
      SynonymsInsectDirect™ , pIEx™-1 Ek/LIC Vector Kit
      References
      Product Information
      Components
      DeclarationpIEx is a trademark of EMD Biosciences, Inc., Madison, WI USA
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      71237 0

      Documentation

      pIEx™-1 Ek/LIC Vector Kit Certificates of Analysis

      TitleLot Number
      71237

      Citations

      Title
    • Kathryn H. Loomis, et al. (2005) InsectDirectTM System: rapid, high-level protein expression and purication from insect cells. Journal of Structural and Functional Genomics 6, 189-194.
    • User Protocols

      Title
      TB163 Ek/LIC Cloning Kits

      Vector Map

      Title
      TB349VM pIEx™-1 Vector Map

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Insect Expression > Insect Expression Vectors & Kits