Millipore Sigma Vibrant Logo

109215 Ziehl-Neelsen carbol-fuchsin solution

for microscopy

Ziehl-Neelsen carbol-fuchsin solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Carbol fuchsin solution

View Products on Sigmaaldrich.com
109215
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
1092150100
Retrieving availability...
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Botol kaca 100 ml
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      1092150500
      Retrieving availability...
      —
      Limited Availability Limited Availability
      In Stock 
      Discontinued
      Limited Quantities Available
      Availability to be confirmed
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          		 Botol plastik 500 ml
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity is a multiple of
          Maximum Quantity is
          Upon Order Completion More Information
          You Saved ()
           
          —
          Request Pricing
          		 —
          1092150507
          Retrieving availability...
          —
          Limited Availability Limited Availability
          In Stock 
          Discontinued
          Limited Quantities Available
          Availability to be confirmed
            Remaining : Will advise
              Remaining : Will advise
              Will advise
              Contact Customer Service
              Contact Customer Service

              		 Botol plastik 500 ml
              Retrieving price...
              Price could not be retrieved
              Minimum Quantity is a multiple of
              Maximum Quantity is
              Upon Order Completion More Information
              You Saved ()
               
              —
              Request Pricing
              		 —
              1092152500
              Retrieving availability...
              —
              Limited Availability Limited Availability
              In Stock 
              Discontinued
              Limited Quantities Available
              Availability to be confirmed
                Remaining : Will advise
                  Remaining : Will advise
                  Will advise
                  Contact Customer Service
                  Contact Customer Service

                  		 Botol plastik 2.5 l
                  Retrieving price...
                  Price could not be retrieved
                  Minimum Quantity is a multiple of
                  Maximum Quantity is
                  Upon Order Completion More Information
                  You Saved ()
                   
                  —
                  Request Pricing
                  		 —
                  Description
                  Catalogue Number109215
                  SynonymsCarbol fuchsin solution
                  DescriptionZiehl-Neelsen carbol-fuchsin solution
                  OverviewZiehl-Neelsen carbolfuchsin solution - for microscopy, is a ready-to use solution used in the Ziehl-Neelsen hot staining for the microscopic investigation of acid-fast bacteria(AFB). The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Cat. no. 100327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use Löfföer's methylene blue solution(Cat. no. 101287) is used for counterstaining. Those three solutions are each 500 ml and are contained in the AFB-color modified staining kit (Cat. No.100497).
                  The 500 ml bottle of Ziehl-Neelsen carbolfuchsin solution provides approx. 250 stainings. Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. Ziehl-Neelsen carbolfuchsin solution - for microscopy is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
                  References
                  Product Information
                  HS Code3212 90 90
                  Quality LevelMQ400
                  Applications
                  Applicationfor microscopy
                  Biological Information
                  Physicochemical Information
                  Density0.99 g/cm3 (20 °C)
                  Flash point47 °C
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  Hazard Pictogram(s) Hazard Pictogram(s)
                  Hazard Pictogram(s)
                  Hazard Pictogram(s)
                  Hazard Pictogram(s)
                  Hazard Statement(s)H226: Cairan dan uap mudah menyala.
                  H314: Menyebabkan kulit terbakar yang parah dan kerusakan mata.
                  H341: Diduga menyebabkan kerusakan genetik.
                  H410: Sangat toksik pada kehidupan perairan dengan efek jangka panjang.
                  Precautionary Statement(s)P210: Jauhkan dari panas/percikan/api terbuka /permukaan yang panas. - Dilarang merokok.
                  P273: Hindarkan pelepasan ke lingkungan.
                  P280: Kenakan sarung tangan pelindung/ pakaian pelindung/ pelindung mata/ pelindung wajah/ perlindungan pendengaran.
                  P303 + P361 + P353: JIKA TERKENA KULIT (atau rambut): Tanggalkan segera semua pakaian yang terkontaminasi. Bilas kulit dengan air.
                  P304 + P340 + P310: JIKA TERHIRUP: Pindahkan korban ke udara segar dan posisikan yang nyaman untuk bernapas. Segera hubungi SENTRA INFORMASI KERACUNAN atau dokter/tenaga medis.
                  P305 + P351 + P338: JIKA TERKENA MATA : Bilas dengan seksama dengan air untuk beberapa menit. Lepaskan lensa kontak jika memakainya dan mudah melakukannya.Lanjutkan membilas.
                  Signal WordBahaya
                  Storage class3 Cairan mudah terbakar
                  WGKWGK 3 sangat berbahaya untuk air
                  Disposal9
                  Senyawa karsinogenik dan senyawa yang dapat terbakar yang berlabel "Very Toxic" (Sangat beracun) atau "Toxic" (beracun) : kategori F. Alkil sulfat bersifat karsinogenik. Berhati-hatilah untuk menghindari penghirupan dan kontak dengan kulit. Untuk menghilangkan keaktifan alkil sulfat, tambahkan tetes per tetes (dari dalam corong tetes) ke dalam larutan amonia pekat dingin-es (Kat. No. 105426), dengan terus diaduk kuat. Sebelum ditempatkan di dalam kategori D, periksa pH dengan indikator pH universal (Kat. No. 105426).
                  Safety Information
                  Categories of dangerdapat terbakar, berbahaya, korosif, mutagenik
                  Product Usage Statements
                  Storage and Shipping Information
                  StorageSimpan pada +15°C hingga +25°C.
                  Packaging Information
                  Transport Information
                  Declaration (railroad and road) ADR, RIDUN 1992 , 3 (6.1), III
                  Declaration (transport by air) IATA-DGRUN 1992 , 3 (6.1), III
                  Declaration (transport by sea) IMDG-CodeUN 1992 , 3 (6.1), III
                  Supplemental Information
                  Specifications
                  Global Trade Item Number
                  Catalogue Number GTIN
                  1092150100 04022536112008
                  1092150500 04022536112015
                  1092150507 04054839116780
                  1092152500 04022536112039

                  Related Products & Applications

                  Alternative Products

                  Catalogue Number Description  
                  100497 AFB-Color dimodifikasi Show Pricing & Availability

                  Accessories (Primary)

                  Catalogue Number Description  
                  100327 Hydrochloric acid in ethanol Show Pricing & Availability
                  101287 Löffler's methylene blue solution Show Pricing & Availability
                  116450 Kit pewarnaan AFB-Color Show Pricing & Availability

                  Accessories (Secondary)

                  Catalogue Number Description  
                  100497 AFB-Color dimodifikasi Show Pricing & Availability

                  Related Products By: Application Facete

                  Bacteria Staining

                  Categories

                  IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Auxiliaries for Bacteriology
                  IVD/OEM Materials & Reagents
                  IVD/OEM Materials & Reagents > Microscopy > Bacteriology > General Bacteriological Staining Solutions
                  IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Acid-Fast Bacteria Staining