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42600 Ultrafree-DA Centrifugal Filter Unit

Polypropylene, 0.5 mL volume, non-sterile, for DNA extraction

Ultrafree-DA Centrifugal Filter Unit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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42600
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      Overview

      Description
      Catalogue Number42600
      Trade Name
      • Ultrafree
      DescriptionUltrafree-DA Centrifugal Filter Unit
      OverviewRecover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin. The kit consists of a pre-assembled filter device with an agarose gel nebulizer, a microcentrifuge vial, and modified TAE gel extraction buffer (dry).

      The device utilizes gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.

      Prepared DNA requires no further purification for most applications, including cloning and radioisotopic or fluorescent DNA sequencing. Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product.

      • Minimal hands-on time
      • 10-minute spin
      • Fully-functional DNA


      Performance
      Typical DNA Recoveries from Agarose Gels

      DNA Size (bp) Percent DNA Recoveries

      100 78
      700 71
      1000 77
      2027 47
      4361 35
      9416 32
      23130 29

      Protocol
      1. Excise the band of interest after routine electrophoretic analysis of PCR products or other DNA through an agarose gel.
      2. Place the gel slice into the pre-assembled device. Note: Protocol is not compatible with low melting point agarose.
      3. Spin at 5,000 x g for 10 minutes to extract gel slurry into filter cup.
      4. DNA can be stored in the capped filtrate vial after discarding the sample filter cup and gel nebulizer.
      Product Information
      HS Code8421 29 90
      Color CodeBlue
      Maximum Operating Temperature50 °C
      Filter TypeMicrofiltration
      Quality LevelMQ400
      Applications
      ApplicationPolypropylene, 0.5 mL volume, non-sterile, for DNA extraction
      Key Applications
      • DNA Extraction
      Application NotesFixed-angle microfuge rotor w/maximum 5,000 x g
      Biological Information
      SterilityNon-Sterile
      Physicochemical Information
      Pore SizeNot Applicable
      Centrifugal ApplicationDNA Gel Extraction
      Dimensions
      Length45 mm
      Diameter10.7 mm
      Filtration Area0.2 cm²
      Volume0.5 mL
      Materials Information
      Chemistry
      • Polypropylene (PP)
      Device Material
      • Polypropylene
      Packaging Information
      Material Size50
      Global Trade Item Number
      Catalogue Number GTIN
      42600 04053252743214