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23-051M UbcH13/Uev1A Protein, active, 250 µg

Active complex of 2 full length, recombinant human enzymes: UbcH13 & Uev1A. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.

UbcH13/Uev1A Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
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23-051M
250 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsSpeciesCascade Component
      Enzyme AssaysHuman E2
      Description
      Catalogue Number23-051M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionUbcH13/Uev1A Protein, active, 250 µg
      Alternate Names
      • UbcH13 - Ubiquitin-conjugating enzyme E2 N
      • Bendless-like ubiquitin-conjugating enzyme
      • Ubiquitin carrier protein N
      • UBE2N
      • Ubc13
      • Uev1A - Ubiquitin-conjugating enzyme E2 variant 1
      • CROC-1
      • UBE2V1
      Background InformationUbiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or a Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate. Typically, a polyubiquitin chain that targets a protein for degradation by the proteasome is linked through lysine-48 (K48) of ubiquitin, however ubiquitin chains linked through other lysines such as K63 have also been found. K63 linked chains are non-degradative and play a role in signalling and protein trafficking. The heterodimeric E2 complex of Ubc13 and Uev1A has been shown to be involved in the formation of K63 linkages in conjunction with a number of E3 ligases including Parkin, CHIP and TRAF6.
      References
      Product Information
      Quality LevelMQ300
      Applications
      ApplicationActive complex of 2 full length, recombinant human enzymes: UbcH13 & Uev1A. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
      Key Applications
      • Enzyme Assays
      Biological Information
      Cascade ComponentE2
      Entrez Gene Number
      Purification MethodPurified using immobilized metal affinity chromatography.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      23-051M 04053252649929

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      Alternative Packsize

      Catalogue Number Description  
      23-051 UbcH13/Uev1A Protein, active, 10 µg Show Pricing & Availability

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      Categories

      Life Science Research > Drug Discovery and Development > Ubiquitin and Proteasome Screening > Ubiquitin E2 Conjugating Enzymes
      Life Science Research > Proteins and Enzymes > Ubiquitin E2 Conjugating Enzymes