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PF019 Sigma-AldrichTIMP-1, Human, Recombinant, CHO Cells

Recombinant, human TIMP-1 expressed in CHO cells.

TIMP-1, Human, Recombinant, CHO Cells MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet
Citations

Synonyms: Tissue Inhibitor of Metalloproteinase-1

Primary Target: Human MMP 1

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Overview

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Description
Overview	Recombinant, human TIMP-1 expressed in CHO cells. Migrates as a 28 kDa protein under reducing conditions on SDS-PAGE.
Catalogue Number	PF019
Brand Family	Calbiochem®
Synonyms	Tissue Inhibitor of Metalloproteinase-1

References
References	Freudenstein, J. et al. 1990. Biochem. Biophys. Res. Comm. 171, 250. Liotta, L. A., and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1, 99. Kodama, S. et al. 1987. Collagen Rel. Res. 7, 341. Docherty, A.J.P. et al. 1985. Nature 318, 66. Nagai, Y., et al. 1984. Jpn. J. Inflam. 4, 123.

Product Information
ATP Competitive	N
Declaration	Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
Form	Lyophilized
Formulation	Lyophilized from ammonium bicarbonate, sodium chloride, sodium phosphate buffer, 100 µg BSA, pH 7.3.
Preservative	None
Reversible	N
Quality Level	MQ100

Applications
Application References	Inhibition Studies Nagai, Y., et al. 1984. Jpn. J. Inflam. 4, 123.
Application Notes	Immunoblotting Standard (20 µl/lane) MMP Inhibition (assay dependent, see comments)
Application Comments	TIMPs bind MMPs in a 1:1 stoichiometric ratio, but due to the variable concentration of MMPs in biological samples the optimal amount of TIMP-1 will be assay dependent and should be determined by titration. It is reported that TIMP-1 inhibits 6.7 nM MMP-1 with an IC₅₀ of 1-5 nM (see application references).

Biological Information
Biological activity	IC₅₀ against native human MMP-1 is ~1-5 nM.
Primary Target	Human MMP 1
Primary Target IC<sub>50</sub>	1-5 nM against human MMP 1
Purity	≥95% by SDS PAGE (prior to lyophilization)

Physicochemical Information
Cell permeable	N

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
PF019-3UG	04055977207934

Documentation

TIMP-1, Human, Recombinant, CHO Cells MSDS

Title
Safety Data Sheet (SDS)

TIMP-1, Human, Recombinant, CHO Cells Certificates of Analysis

Title	Lot Number
PF019	Enter Lot Number

References

Reference overview
Freudenstein, J. et al. 1990. Biochem. Biophys. Res. Comm. 171, 250. Liotta, L. A., and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1, 99. Kodama, S. et al. 1987. Collagen Rel. Res. 7, 341. Docherty, A.J.P. et al. 1985. Nature 318, 66. Nagai, Y., et al. 1984. Jpn. J. Inflam. 4, 123.

Citations

Title

William J. Lane, et al. (2000) Stromal-derived factor 1-induced megakaryocyte migration and platelet production is dependent on matrix metalloproteinases. Blood 96, 4152-4159.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	06-September-2007 JSW
Synonyms	Tissue Inhibitor of Metalloproteinase-1
Application	Immunoblotting Standard (20 µl/lane) MMP Inhibition (assay dependent, see comments)
Description	Recombinant, human TIMP-1 expressed in CHO cells. Migrates as a ~28 kDa protein by SDS-PAGE. The activation and activity of matrix metalloproteinases (MMPs) are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-1 is a ~28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models.
Background	The activation and activity of matrix metalloproteinases (MMPs) are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-1 is a 28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models.
Form	Lyophilized
Formulation	Lyophilized from ammonium bicarbonate, sodium chloride, sodium phosphate buffer, 100 µg BSA, pH 7.3.
Recommended reaction conditions	Immunoblotting Standard Useful as an immunoblotting standard; use at 20 µl/lane for most applications. MMP Inhibition Assays TIMPs bind MMPs in a 1:1 stoichiometric ratio, but due to the variable concentration of MMPs in biological samples the optimal amount of TIMP-1 will be assay dependent and should be determined by titration. It is reported that TIMP-1 inhibits 6.7 nM MMP-1 with an IC₅₀ of 1-5 nM (see application references).
Purity	≥95% by SDS PAGE (prior to lyophilization)
Biological activity	IC₅₀ against native human MMP-1 is ~1-5 nM.
Solubility	Reconstitute with PBS to the desired concentration and mix gently until completely dissolved.
Preservative	None
Comments	TIMPs bind MMPs in a 1:1 stoichiometric ratio, but due to the variable concentration of MMPs in biological samples the optimal amount of TIMP-1 will be assay dependent and should be determined by titration. It is reported that TIMP-1 inhibits 6.7 nM MMP-1 with an IC₅₀ of 1-5 nM (see application references).
Storage	-20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Toxicity	Standard Handling
References	Freudenstein, J. et al. 1990. Biochem. Biophys. Res. Comm. 171, 250. Liotta, L. A., and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1, 99. Kodama, S. et al. 1987. Collagen Rel. Res. 7, 341. Docherty, A.J.P. et al. 1985. Nature 318, 66. Nagai, Y., et al. 1984. Jpn. J. Inflam. 4, 123.
Citation	William J. Lane, et al. (2000) Stromal-derived factor 1-induced megakaryocyte migration and platelet production is dependent on matrix metalloproteinases. Blood 96, 4152-4159.
Application references	Inhibition Studies Nagai, Y., et al. 1984. Jpn. J. Inflam. 4, 123.

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