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AC111S Sheep Anti-Mouse IgG Antibody, Species absorbed, Cy5 Conjugate

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AC111S
400 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MICCShCy5Polyclonal Antibody
      Description
      Catalogue NumberAC111S
      DescriptionSheep Anti-Mouse IgG Antibody, Species absorbed, Cy5 Conjugate
      Background InformationImmunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.
      Product Information
      FormatCy5
      PresentationPurified sheep polyclonal antibody in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationSheep Anti-Mouse IgG Antibody, Species absorbed, Cy5 Conjugate is an antibody against Sheep Mouse IgG for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Biological Information
      ImmunogenPurified mouse IgG.
      ConcentrationPlease refer to lot specific datasheet.
      HostSheep
      SpecificityBased on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with whole molecule mouse IgG (IgG2>IgG2a>IgG3>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reactivity toward chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Purification MethodFractionated by octanoic acid and ammonium sulfate cut, and then depleted with rat IgG column and mouse IgA column prior to Cy3 conjugation.
      UniProt Number
      Molecular Weight~150 kDa observed.
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: Performance of this Cy5-conjugated secondary antibody has been validated by indirect immunofluorescent labelling (1:250 dilution) of A431 cells following a mouse monoclonal anti-H3K27me3 primary antibody (Cat. No. 05-1951).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size400 µL
      Global Trade Item Number
      Catalogue Number GTIN
      AC111S 04055977344110