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540411 Puromycin, Dihydrochloride, Cell Culture-Tested - CAS 58-58-2 - Calbiochem

Puromycin, CAS 58-58-2, is a protein synthesis inhibitor that causes premature release of nascent polypeptide chains.

Puromycin, Dihydrochloride, Cell Culture-Tested - CAS 58-58-2 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: protein synthesis
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      Overview

      Replacement Information

      Key Spec Table

      CAS #Empirical Formula
      58-58-2C₂₂H₂₉N₇O₅ · 2HCl
      Description
      OverviewProtein synthesis inhibitor. Inhibits translation by causing premature release of nascent polypeptide chains. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
      Catalogue Number540411
      Brand Family Calbiochem®
      References
      ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
      Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
      de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
      Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.
      Product Information
      CAS number58-58-2
      FormWhite to off-white solid
      Hill FormulaC₂₂H₂₉N₇O₅ · 2HCl
      Chemical formulaC₂₂H₂₉N₇O₅ · 2HCl
      Quality LevelMQ100
      Applications
      Biological Information
      Primary Targetprotein synthesis
      Purity≥98% by HPLC
      Physicochemical Information
      ContaminantsEndotoxin: ≤10 EU/mg dry weight
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSAU7355000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage -20°C
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      540411 0

      Documentation

      Puromycin, Dihydrochloride, Cell Culture-Tested - CAS 58-58-2 - Calbiochem MSDS

      Title

      Safety Data Sheet (SDS) 

      Puromycin, Dihydrochloride, Cell Culture-Tested - CAS 58-58-2 - Calbiochem Certificates of Analysis

      TitleLot Number
      540411

      References

      Reference overview
      Chow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
      Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
      de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
      Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-April-2011 JSW
      DescriptionAn aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor. Resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the "A site" of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Useful for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Recommended range for selection of transfected mammalian cells ranges from 1 to 30 µg/ml.
      FormWhite to off-white solid
      CAS number58-58-2
      RTECSAU7355000
      Chemical formulaC₂₂H₂₉N₇O₅ · 2HCl
      Purity≥98% by HPLC
      ContaminantsEndotoxin: ≤10 EU/mg dry weight
      SolubilityH₂O (50 mg/ml) and Methanol (20 mg/ml)
      Storage Protect from moisture
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Harmful
      Merck USA index14, 7943
      ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
      Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
      de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
      Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.