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411550 Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate

This Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific.

Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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411550
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411550-500UG
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      Description
      Catalogue Number411550
      Brand Family Calbiochem®
      References
      ReferencesHamilton, R.G. 1993. Clin. Chem. 39, 1988.
      Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth. 158, 107.
      Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275.
      Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
      Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem. 22, 1084.
      Product Information
      FormLiquid
      FormulationIn PBS, 10 mg/ml BSA.
      PreservativeNone
      Quality LevelMQ200
      Applications
      Application ReferencesEnzyme Immunoassay Hamilton, R.G., et al. 1987. Hybridoma 6, 204. Cross-Reactivity, Myeloma Proteins Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275. Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
      Key Applications Enzyme Immunoassay
      Application NotesEnzyme Immunoassay (1:1000, see application references)
      Application CommentsAntibody should be titrated for optimal results in individual systems.

      Cross-reactivity by ELISA against human myeloma proteins:
      Human IgG1: 100%
      Human IgG2: 100%
      Human IgG3: 100%
      Human IgG4: 100%
      Human IgM: <0.2%
      Human IgA: <0.1%
      Human IgE: <0.1%

      Cross-reactivity by ELISA against chimeric antibodies:
      Human IgG1: 100%
      Human IgG2: 100%
      Human IgG3: 100%
      Human IgG4: 100%
      Human IgM: <0.01%
      Human IgA: <0.01%
      Human IgE: <0.01%

      There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
      Biological Information
      CloneHP6043
      HostMouse
      IsotypeIgG
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
      Global Trade Item Number
      Catalogue Number GTIN
      411550-500UG 07790788060275