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444256 MMP Substrate III, Fluorogenic - Calbiochem

A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination.

MMP Substrate III, Fluorogenic - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: DABCYL-GABA-PQGL-E(EDANS)-AK-NH₂, TNO211

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444256
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Overview

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444256-500UG
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      Description
      OverviewA specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity [kcat/Km = 619,000 M-1s-1 for MMP-2, 206,000 M-1s-1 for MMP-9, 40,000 M-1s-1 for MMP-3, and 21,000 M-1s-1 for MMP-1; at 37°C, pH 7.6 ]. Exhibits a high degree of sensitivity that is not affected by optical disturbances in biological media. Also useful for MMP activity measurements in synovial fluid and culture medium.
      Catalogue Number444256
      Brand Family Calbiochem®
      SynonymsDABCYL-GABA-PQGL-E(EDANS)-AK-NH₂, TNO211
      References
      ReferencesBeekman, B., et al. 1996. FEBS Lett 390, 221.
      Product Information
      ATP CompetitiveN
      FormRed lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₆₃H₈₈N₁₆O₁₄S
      Chemical formulaC₆₃H₈₈N₁₆O₁₄S
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetDetection of MMP activity
      Purity≥97% by HPLC
      Physicochemical Information
      Cell permeableN
      Emission max.
      Excitation max.
      Peptide SequenceDABCYL-GABA-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH₂
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      444256-500UG 04055977186543

      Documentation

      MMP Substrate III, Fluorogenic - Calbiochem MSDS

      Title

      Safety Data Sheet (SDS) 

      MMP Substrate III, Fluorogenic - Calbiochem Certificates of Analysis

      TitleLot Number
      444256

      References

      Reference overview
      Beekman, B., et al. 1996. FEBS Lett 390, 221.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-August-2008 RFH
      SynonymsDABCYL-GABA-PQGL-E(EDANS)-AK-NH₂, TNO211
      DescriptionA fluorescence energy transfer (FRET) peptide substrate with MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity (kcat/Km = 619,000 M-1s-1 for MMP-2, 206,000 M-1s-1 for MMP-9, 40,000 M-1s-1 for MMP-3, 21,000 M-1s-1 for MMP-1; pH = 7.6 at 37°C). Exhibits a high degree of sensitivity, which is not affected by optical disturbances in biological media. Excitation max.: ~340 nm; emission max.: ~485 nm.
      FormRed lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₆₃H₈₈N₁₆O₁₄S
      Peptide SequenceDABCYL-GABA-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH₂
      Purity≥97% by HPLC
      SolubilityDMSO and Tris buffered saline (pH 7.6)
      Storage Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesBeekman, B., et al. 1996. FEBS Lett 390, 221.