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105387
Sigma-AldrichLeishman's eosin methylene blue solution modified
Leishman's eosin methylene blue solution modified MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Catalogue Number
105387
Description
Leishman's eosin methylene blue solution modified
Overview
The staining method according to Leishman is one of the standard techniques in hematological diagnostic procedures. Like Wright's eosin methylene blue solution and May-Grünwald's eosin methylene blue solution,this ready-to-use solution is used for staining of blood and bone marrow smears and clinical cytological specimens. The typical resulting color of cell nuclei is red to purple, which depends on the buffer tablets (Buffer tablets pH 7.2 or Buffer tablets pH 6.8). The staining of the nuclei is due of the molecular interaction of the Eosin Y dye and a complex of Azur B with DNA. Both dyes assemble to an Eosin Y - Azur B-DNA complex and the intensity of the resulting stain depends on the content of Azur B and the ratio of Azur B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. A 500 mL bottle of Leishman's eosin methylene blue solution are sufficient for staining up to 1600 slides in Hellendahl cells.As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
H225: Cairan dan uap amat mudah menyala. H301 + H311 + H331: Toksik bila tertelan, terkena kulit atau bila terhirup. H317: Dapat menyebabkan reaksi alergi pada kulit. H370: Menyebabkan kerusakan pada organ.
Precautionary Statement(s)
P210: Jauhkan dari panas/percikan/api terbuka /permukaan yang panas. - Dilarang merokok. P233: Jaga wadah tertutup rapat. P280: Kenakan sarung tangan pelindung/ pakaian pelindung/ pelindung mata/ pelindung wajah/ perlindungan pendengaran. P301 + P310: JIKA TERTELAN: Segera hubungi SENTRA INFORMASI KERACUNAN atau dokter/tenaga medis. P303 + P361 + P353: JIKA TERKENA KULIT (atau rambut): Tanggalkan segera semua pakaian yang terkontaminasi. Bilas kulit dengan air. P304 + P340 + P311: JIKA TERHIRUP: Pindahkan korban ke udara segar dan posisikan yang nyaman untuk bernapas. Hubungi SENTRA INFORMASI KERACUNAN atau dokter/enaga medis. P403 + P233: Simpan di tempat berventilasi baik. Jaga wadah tertutup kedap/rapat.
Signal Word
Bahaya
Storage class
3 Cairan mudah terbakar
WGK
WGK 2 membahayakan air secara signifikan
Disposal
1 Pelarut organik bebas halogen: wadah A
Safety Information
Categories of danger
mudah terbakar, Toksik
Product Usage Statements
Storage and Shipping Information
Storage
Simpan pada +15°C hingga +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RID
UN 1230 , 3 (6.1), II
Declaration (transport by air) IATA-DGR
UN 1230 , 3 (6.1), II
Declaration (transport by sea) IMDG-Code
UN 1230 , 3 (6.1), II
Supplemental Information
Specifications
Suitability for microscopy (Blood smear)
passes test
Erythrocytes
reddish
Nuclei
violet
Eosinophilic granules
brick-red
Neutrophilic granules
light violet
Basophilic granules
dark violet
Lymphocyte cytoplasmn
blue
Monocyte cytoplasm
grey-blue
Thrombocytes
violet
Global Trade Item Number
Catalogue Number
GTIN
1053870500
04022536063911
Documentation
Leishman's eosin methylene blue solution modified MSDS
