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71086 KOD Hot Start DNA Polymerase

High fidelity DNA polymerase designed for accurate PCR amplification of long strand and GC- rich DNA templates for cloning and cDNA amplification applications.

KOD Hot Start DNA Polymerase MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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71086
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                  Description
                  OverviewPCR involves replication of a DNA template by a thermostable DNA polymerase. The processivity, specificity, and fidelity of the polymerase enzyme used can influence the efficiency, reproducibility, and yield of the PCR reaction. High-fidelity PCR, utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. Fidelity is critical when accurate sequence amplification of the gene target is needed, for example, when direct sequencing or cloning for downstream protein expression. Unwarranted mutation could severely impact your studies. Our analysis has shown that KOD enzymes are an easy choice for fast, accurate and high-yielding PCR. EMD Millipore's molecular biologists work to develop and formulate polymerases offering the highest specificity, fidelity and yield during PCR amplification. In addition, optimized buffer compositions, convenient master mixes and cycling parameters provide additional ease of use and data reproducibility. KOD Hot Start DNA Polymerase* is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3'→5' exonuclease activities at ambient temperatures (Mizuguchi 1999). KOD Hot Start amplifies genomic DNA templates up to 21 kb including GC-rich genes for PCR applications. KOD Hot Start combines the high fidelity, fast extension speed, and outstanding processivity of KOD with the high specificity of an antibody-mediated hot start. Non-specific amplification is reduced because mispriming events that can occur during setup and initial temperature increase are avoided. In addition, primer degradation during setup at room temperature due to exonuclease activity is effectively inhibited. KOD Hot Start DNA Polymerase generates blunt-ended PCR products suitable for cloning with the Novagen Perfectly Blunt® and LIC Vector Kits.

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                  Source Recombinant Thermococcus kodakaraensis KOD1 DNA polymerase expressed in E. coli
                  Concentration 1.0 U/µl
                  Nicking activity None detected
                  Amplification effiency Functional PCR; inhibition of activity at 21°C verified
                  Storage –20°C

                  *Manufactured by Toyobo and distributed by EMD. Not available in Japan.

                  Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

                  Catalogue Number71086 Brand Family Novagen®
                  Features and benefits
                  • Highest accuracy, yield, and processivity of commercially available proofreading DNA polymerases
                  • Amplifies genomic DNA templates up to 12 kbp
                  • Amplifies plasmid and lambda DNA templates up to 21 kbp
                  • Successfully amplifies GC-rich sequences
                  • Eliminates mispriming and primer-dimer formation
                  • Convenient room-temperature setup compatible with automation
                  • Optimal KOD Hot Start Buffer for PCR performance over a wide range of targets
                  References
                  ReferencesMizuguchi, H., et al. 1999. J. Biochem. (Tokyo) 126, 762. Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem. 66 (10), 2194. Fujii, S., et al. 1999. J. Mol. Biol. 289, 835.
                  Product Information
                  Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [<Sup>3</Sup>H]dTTP), and 150 µg/ml activated calf thymus DNA.
                  Components
                  DeclarationManufactured by Toyobo and distributed by EMD. Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
                  Quality LevelMQ200
                  Applications
                  Biological Information
                  Physicochemical Information
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  Safety Information
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Shipped with Blue Ice or with Dry Ice
                  Toxicity Standard Handling
                  Storage -20°C
                  Do not freeze Ok to freeze
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications
                  Global Trade Item Number
                  Catalogue Number GTIN
                  71086-3 07790788053017
                  71086-4 07790788060268
                  71086-5 04055977271720
                  71086-EA 04055977297218

                  Documentation

                  KOD Hot Start DNA Polymerase Certificates of Analysis

                  TitleLot Number
                  71086

                  References

                  Reference overview
                  Mizuguchi, H., et al. 1999. J. Biochem. (Tokyo) 126, 762. Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem. 66 (10), 2194. Fujii, S., et al. 1999. J. Mol. Biol. 289, 835.

                  Citations

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                  Title
                  TB341 KOD Hot Start DNA Polymerase

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                  Life Science Research > Genomic Analysis > DNA Preparation & Cloning > PCR & RT-PCR Kits & Reagents