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IHCR2025-6 IHC Select® Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3

IHC Select Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3 is an antibody against Cytokeratin AE1/AE3 (Pan cytokeratins) for use in IH(P).

IHC Select® Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytokeratins
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IHCR2025-6
6 mL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberIHCR2025-6
      Trade Name
      • IHC Select
      • Chemicon
      DescriptionIHC Select® Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3
      OverviewKeratins are a group of water-insoluble proteins that form monofilaments, a class of intermediate filament. These filaments form part of the cytoskeletal complex in epidermis and in most other epithelial tissues. Nineteen human epithelial keratins are resolved with two-dimensional gels electrophoresis (Moll, R., 1982). These can be divided into acid (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40 kD. The basic keratins have molecular weights of 65-67, 64, 59, 58, 56, and 52 kD. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state (Sun, T.T., 1984; Cooper, D., 1985; Sun, T. T., 1985 ). The 56.5/65-67 kD pair is present in keratinized (differentiated) epidermis. The 55/64 kD pair is characteristic of normal (corneal-type) epithelial differentiation (Moll, R., 1982; Sun, T.T, 1984). The 51/59 kD pair is characteristic of the stratified squamous epithelial of internal organism such as esophagus and tongue (Moll, R., 1982; Cooper, D., 1985). The 51/58 kD pair is a keratinocyte marker; this pair is present in almost all stratified epithelia irrespective of the state of cellular stratification (Moll, R., 1982; Sun, T.T., 1984). The 48/56 kD pair is characteristic of hyper-proliferative (de-differentiated) keratinocytes (Moll, R., 1982; Weiss, R.A., 1984). The 45/52 kD pair and to a lesser extent, the 46/54 kD pair are characteristic of simple epithelia (Moll, R., 1982). The 40 kD keratin is present in most epithelia except adult epidermis (Moll, R., 1982).
      References
      Product Information
      FormatPurified
      PresentationPurified monoclonal mouse antibody supplied in liquid format diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% Kathon as preservative.
      Quality LevelMQ100
      Applications
      ApplicationIHC Select Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3 is an antibody against Cytokeratin AE1/AE3 (Pan cytokeratins) for use in IH(P).
      Key Applications
      • Immunohistochemistry (Paraffin)
      Application NotesAntibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

      Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

      Incubation: 10 minutes with IHC Select® Detection Kits.

      Cytokeratin AE1/AE3 has been prediluted for use as the primary antibody with Chemicon's IHC Select® Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier's IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR2025-6.
      Biological Information
      ImmunogenHuman epidermal keratin
      CloneAE1/AE3
      HostMouse
      SpecificityAnti-Keratin AE1 recognizes the 56.5, 50, 50', 48, and 40 kD keratins of the acidic subfamily. Anti-keratin AE3 recognizes all members of the basic subfamily. AE1/AE3 reacts with both acidic and basic keratins. Staining is localized to the cytoplasm. AE1/AE3 specifically binds to antigens located in the cytoplasm of normal epithelia. Reacts with cells of epithelial origin including simple and stratified epithelia and epidermis.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C . Refer to vial for expiration dating.
      Packaging Information
      Material Size6 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      IHCR2025-6 04053252627804

      Documentation

      IHC Select® Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3 MSDS

      Title

      Safety Data Sheet (SDS) 

      IHC Select® Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3 Certificates of Analysis

      TitleLot Number
      MILLIPORE IHC SELECT RESEARCH PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL - 3227854 3227854
      MILLIPORE IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL MONOCLONAL ANTIBODY - 1977809 1977809
      MILLIPORE IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL MONOCLONAL ANTIBODY - 2001344 2001344
      MILLIPORE IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL MONOCLONAL ANTIBODY - 2059294 2059294
      MILLIPORE IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL MONOCLONAL ANTIBODY - 2192722 2192722
      MILLIPORE IHC SELECT RESEARCH® PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL - 3071900 3071900
      MILLIPORE IHC SELECT RESEARCH® PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL - 3160117 3160117
      MILLIPORE IHC SELECT RESEARCH® PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL MONOCLONAL ANTIBODY 2909552
      MILLIPORE IHC SELECT RESEARCH® PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL MONOCLONAL ANTIBODY 3008184
      MILLIPORE IHC SELECT RESEARCH -2800503 2800503
      Show more

      References

      Reference overviewPub Med ID
      Galanin modulates the neural niche to favour perineural invasion in head and neck cancer.
      Scanlon, CS; Banerjee, R; Inglehart, RC; Liu, M; Russo, N; Hariharan, A; van Tubergen, EA; Corson, SL; Asangani, IA; Mistretta, CM; Chinnaiyan, AM; D'Silva, NJ
      Nat Commun  6  6885  2015

      Show Abstract       		25917569 25917569

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      Categories

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