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PF039 GDNF, Rat, Recombinant, S. frugiperda

GDNF, Rat, Recombinant, S. frugiperda MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rrGDNF, Glial Derived Neurotrophic Factor

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      Overview

      Replacement Information

      Key Spec Table

      Purity
      ≥97% by SDS-PAGE
      Description
      Overview

      This product has been discontinued.



      Recombinant, rat glial derived neurotrophic factor expressed in S. frugiperda insect cells. Native GDNF exists as a disulfide homodimeric glycoprotein and is a novel member of the TGF-β superfamily. Titrate the protein for optimal results in individual systems. Immbolized rrGFRa/Fc at 2 µg/ml (100 µl/neu) can bind GDNF at a linear range of 0.1-10 ng/ml.
      Catalogue NumberPF039
      Brand Family Calbiochem®
      SynonymsrrGDNF, Glial Derived Neurotrophic Factor
      References
      ReferencesDurbec, P., et al. 1996. Nature 381, 789.
      Jing, s., et al. 1996. Cell 85, 1113.
      Treanor, J.J.S., et al. 1996. Nature 382 80.
      Trupp, M., et al. 1996. Nature 381, 785.
      Hudson, J., et al. 1995. Brain Res. Bull. 36, 425.
      Tomac, A., et al. 1995. Nature 373, 335.
      Lin, L.-F.H., et al. 1993. Science 260, 1130.
      Product Information
      FormLyophilized
      FormulationLyophilized from 35% Acetonitrile, 0.1% TFA, 50 µg BSA/µg GDNF.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 1-3 ng/ml as measured by the ability to support the survival and stimulate neurite outgrowth of cultured embryonic chick dorsal root ganglia. Immbolized rrGFRα/Fc at 2 µg/ml (100 µl/neu) can bind GDNF at a linear range of 0.1-10 ng/ml.
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤1 EU/µg GDNF
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PF039 0

      Documentation

      GDNF, Rat, Recombinant, S. frugiperda MSDS

      Title

      Safety Data Sheet (SDS) 

      GDNF, Rat, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      PF039

      References

      Reference overview
      Durbec, P., et al. 1996. Nature 381, 789.
      Jing, s., et al. 1996. Cell 85, 1113.
      Treanor, J.J.S., et al. 1996. Nature 382 80.
      Trupp, M., et al. 1996. Nature 381, 785.
      Hudson, J., et al. 1995. Brain Res. Bull. 36, 425.
      Tomac, A., et al. 1995. Nature 373, 335.
      Lin, L.-F.H., et al. 1993. Science 260, 1130.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2008 RFH
      SynonymsrrGDNF, Glial Derived Neurotrophic Factor
      DescriptionRecombinant, rat glial derived neurotrophic factor expressed in S. frugiperda insect cells. GDNF is a recombinant rat protein whose DNA sequence was inserted in a baculovirus expression vector and expressed in Sf 21 insect cells. The mature rat GDNF is a disulfide linked dimeric protein containing two 134 amino acid with a predicted molecular weight of 15 kDa for the monomer. Useful for proliferation studies. Glial cell line-derived neurotrophic factor (GDNF) was initially isolated based upon its ability to maintain embryonic midbrain dopaminergic neuron survival in vitro, and additional in vivo studies have provided support for the role of GDNF as a trophic factor in both the central and peripheral nervous systems. GDNF is structurally related to the TGF-β superfamily, and has been shown to interact with the GDNF receptor (GDNFRα). GDNFRα is associated with the cell membrane via a glycosyl-phosphatidylinositol (GPI) linkage, and together with Ret forms a functional receptor complex.
      FormLyophilized
      FormulationLyophilized from 35% Acetonitrile, 0.1% TFA, 50 µg BSA/µg GDNF.
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: ≤1 EU/µg GDNF
      Biological activityED₅₀ = 1-3 ng/ml as measured by the ability to support the survival and stimulate neurite outgrowth of cultured embryonic chick dorsal root ganglia. Immbolized rrGFRα/Fc at 2 µg/ml (100 µl/neu) can bind GDNF at a linear range of 0.1-10 ng/ml.
      SolubilityReconstitute in sterile PBS containing ≥0.1% HSA or BSA to a final concentration of ≥ 10 µg/ml.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C.
      Toxicity Standard Handling
      ReferencesDurbec, P., et al. 1996. Nature 381, 789.
      Jing, s., et al. 1996. Cell 85, 1113.
      Treanor, J.J.S., et al. 1996. Nature 382 80.
      Trupp, M., et al. 1996. Nature 381, 785.
      Hudson, J., et al. 1995. Brain Res. Bull. 36, 425.
      Tomac, A., et al. 1995. Nature 373, 335.
      Lin, L.-F.H., et al. 1993. Science 260, 1130.