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SCT152 Firefly/Renilla Dual Luciferase Assay

Flash-type dual luciferase assay that allows measurement of both Firefly and Renilla luciferase activity in the same sample with high sensitivity and linearity.

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SCT152
1 kit  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCT152
      DescriptionFirefly/Renilla Dual Luciferase Assay
      OverviewFirefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalizing transfection control for firefly luciferase assays.

      The Firefly/Renilla Dual Luciferase Assay allows measurement of both Firefly and Renilla luciferase activity in the same sample with high sensitivity and linearity. Firefly luciferase activity is measured first, then Renilla Luciferase Assay Buffer 2.0 is added to simultaneously quench firefly luciferase activity and measure Renilla luciferase activity. Renilla Luciferase Assay Buffer 2.0 quenches the firefly luciferase activity to the level of untransfected cells, allowing sequential measurement of firefly and Renilla luciferase activity in the same sample. This is a flash-type assay that requires luminescence to be measured immediately after adding the detection reagents to the luciferase sample. Firefly signal decays over the course of about 12 minutes, while Renilla signal decays over the course of about 2 minutes, although this may vary depending on enzyme levels.
      Alternate Names
      • Firefly Luciferase Assay
      • Dual Luciferase Assay
      • Bioluminescent Assay
      References
      Product Information
      Components
      • 1) 1X Passive Luciferase Lysis Buffer 2.0 (CS224514): 15 mL
      • 2) Firefly Luciferase Assay Buffer 2.0 (CS224591): 15 mL
      • 3) D-Luciferin (CS224551): 3 X 1 mg
      • 4) Renilla Luciferase Assay Buffer 2.0 (CS224580): 15 mL
      • 5) Aquaphile™ Coelenterazine (CS224581): 3 X 200 µg
      Detection methodChemiluminescence
      PresentationLiquid
      Applications
      ApplicationFlash-type dual luciferase assay that allows measurement of both Firefly and Renilla luciferase activity in the same sample with high sensitivity and linearity.
      Key Applications
      • Cell Based Assays
      Application NotesFirefly Luciferase Assay
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore Firefly/Renilla Dual Luciferase Assay at -80°C. Firefly and Renilla Assay Buffers are stable at -80°C for at least six months from date of receipt. Other components are stable at -20°C or below for at least six months from date of receipt. Kit components and stock solutions of D-Luciferin and Aquaphile Coelenterazine in water are stable to at least 5 freeze/thaw cycles.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT152 04054839566042