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CHR103 Digitonin solution- high purity

CHR103
5 X 1 mL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberCHR103
      DescriptionDigitonin solution- high purity
      Background InformationDigitonin is a non-ionic steroid saponin derived from Digitalis purpurea. Historically, digitonin has been used to solubilize receptors and permeabilize membranes for sample preparation and analysis. Digitonin interacts with cholesterol creating a digitonin-cholesterol complex, which leads to subsequent removal of cholesterol and cellular membrane permeabilization. Therefore, cellular compartments composed of low amounts of cholesterol are protected from digitonin effects, particularly the nuclear membrane.

      Due to digitonin’s mechanism of action, many other epigenetic applications utilize digitonin for nuclear extraction. For example, assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-Seq) is a valuable method to map open chromatin sites and assess the epigenetic landscape. In combination with other detergents, a low concentration of digitonin is employed to isolate the nuclear pellet followed by digestion of the nuclei with an increased concentration of digitonin5. In our ChIC (Chromatin Immuno-Cleavage) Kits (part #s: CHR100-102), digitonin is utilized for antibody targeting of micrococcal nuclease for chromatin tagging.
      References
      Product Information
      Components
      • 5 x 1 ml 2% ( (weight/volume) Digitonin in DMSO.
      PresentationEach vial contains 20mg high purity Digitonin disolved in 1ml DMSO.
      Applications
      Biological Information
      Concentration2% Digitonin in DMSO
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions-20°C
      Packaging Information
      Material Size5 X 1 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CHR103 04061841034650