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SCM302 Definitive Endoderm Induction Medium

Ready-to-use serum-free media for the efficient differentiation of human pluripotent stem cells (ES/iPSCs) to CXCR4+/c-Kit+/Sox-17+/FOXA2+ definitive endoderm (DE) cells.

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SCM302
50 ml  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCM302
      DescriptionDefinitive Endoderm Induction Medium
      Background InformationThe definitive endoderm generates the epithelial lining of the respiratory and digestive tracts as well as cell of the thyroid, thymus, lungs, liver and pancreas. The definitive endoderm induction medium (SCM302) is a defined, animal component-free, ready-to-use serum-free medium for efficient differentiation of human pluripotent stem cells (ES/iPSCs) to definitive endoderm (DE) cells.

      The 3-day differentiation protocol is simple and has been validated on multiple human iPS cell lines. Differentiated cells express high levels (>85%) of endoderm markers including CXCR4, c-Kit, Sox-17 and FOXA2 and can be further differentiated into specialized endodermal cell lineages. Definitive Endoderm Induction Medium can be used to generate 3D organoids such as hiPS derived colon (Cat. No. SCC300) and lung organoids.

      References
      • Hay D, et al. (2008) Highly efficient differentiation of hESCs to functional hepatic endoderm requires ActivinA and Wnt3a signaling. PNAS 26(105):12301-12306.
      • Rezania A, et al. (2012) Maturation of human embryonic stem cell-derived pancreatic progenitors into functional islets capable of treating pre-existing diabetes in mice. Diabetes 61:2016-2029.
      References
      Product Information
      Applications
      ApplicationReady-to-use serum-free media for the efficient differentiation of human pluripotent stem cells (ES/iPSCs) to CXCR4+/c-Kit+/Sox-17+/FOXA2+ definitive endoderm (DE) cells.
      Key Applications
      • Stem Cell Culture
      • Cell Culture
      Biological Information
      Media FormLiquid
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Appearance: Clear liquid/no particulates
      • Sterility Tested: No growth
      • Endotoxin: <2 EU/mL
      • pH: 7.0-7.4
      • Mycoplasma: Negative
      • Functional Assay: >85% CXCR4+ c-Kit+ double positive after 3 days of induction.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store at -20ºC. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly and aliquot into smaller volumes (i.e. 10 mL), use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at -20°C until the expiry date.
      Packaging Information
      Material Size50 ml
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCM302 04054839986499