Millipore Sigma Vibrant Logo

69182 DNase I, RNase free

For applications in which maintenance of RNA integrity is critical

DNase I, RNase free MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: RNase-free DNase I

View Products on Sigmaaldrich.com
69182
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
69182-3
Retrieving availability...
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Botol kaca 1000 u
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      Description
      Overview

      RNase-free DNase I digests either single- or double-stranded DNA, producing a mixture of mono- and oligonucleotides. Purified to be free of RNase, this preparation is qualified for applications in which maintenance of the integrity of RNA is critical. The enzyme selectively degrades DNA in the presence of RNA and can be used to remove DNA template following in vitro transcription reactions. This enzyme is also useful in other applications such as DNase footprinting and nick translation. For applications requiring the use of DNase I for random double-stranded cleavage of DNA, try DNase I, ds Qualified.
      Catalogue Number69182
      Brand Family Novagen®
      SynonymsRNase-free DNase I
      References
      Product Information
      Unit of DefinitionOne unit will degrade 1 μg DNA in 10 minutes at 37°C. The reaction mixture (50 µl) contains 80 mM HEPES pH 7.5, 10 mM NaCl, 5 mM MgCl₂, 10 mM DTT, 1 µg plasmid DNA, and enzyme.
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      69182-3 07790788055844

      Documentation

      DNase I, RNase free MSDS

      Title

      Safety Data Sheet (SDS) 

      DNase I, RNase free Certificates of Analysis

      TitleLot Number
      69182

      Citations

      Title
    • K. M. Hallamaa, G. F. Browning and S. L. Tang. (2006) Lipoprotein multigene families in Mycoplasma pneumoniae. Journal of Bacteriology 188, 5393-5399.
    • G. Ross, et al. (2005) Tuberoinfundibular peptide of 39 residues: a new mediator of cardiac function via nitric oxide production in the rat heart. Endocrinology 146, 2221-2228.
      		•