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17-500 Catch and Release® v2.0 Reversible Immunoprecipitation System

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17-500
1 kit  50 immunoprecipitations
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      Overview

      Key Spec Table

      Species ReactivityKey Applications
      AIP, IPK, WB
      Description
      Catalogue Number17-500
      Brand Family Upstate
      Trade Name
      • Catch & Release
      • Upstate
      DescriptionCatch and Release® v2.0 Reversible Immunoprecipitation System
      OverviewFeatures & Benefits:
      Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
      Convenient: spin-column format dramatically simplifies sample handling
      Reversible: simple elution of antigen:antibody complex in either native or denatured form



      Use:
      This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP's, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release® is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
      Product Information
      Components
      • Catch and Release® Wash Buffer, 10X (Cat.# 20-210)
      • Catch and Release® Non-denaturing Elution Buffer, 4X (Cat.# 20-209)
      • Catch and Release® Denaturing Elution Buffer, 1X (Cat.# 20-284)
      • 20-285
      • 20-216
      • Catch and Release® Capture Tubes
      Quality LevelMQ100
      Applications
      ApplicationCatch & Release v2.0 Reversible Immunoprecipitation System to purify both native & denatured proteins using a variety of antibodies & sample types. Comes in a package of 5 immunoprecipitations.
      Key Applications
      • Immunoprecipitation
      • IP-Kinase Assay
      • Western Blotting
      Application NotesCatch and Release® v2.0 should not be used with proteins containing a 6-His tag.
      Biological Information
      Species Reactivity
      • All
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoprecipitation.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsAll components to be stored at 4°C except the Catch and Release® Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.
      Packaging Information
      Material Size1 kit
      Material Package50 immunoprecipitations
      Global Trade Item Number
      Catalogue Number GTIN
      17-500 04053252323119