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218790 Caspase-1 Assay Kit, Colorimetric

218790
  
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      Overview

      Key Spec Table

      Detection Methods
      Colorimetric
      Description
      OverviewThis kit provides a simple and convenient assay for measuring the activity of caspase-1 and related caspases that recognize the sequence YVAD. The assay is based on the spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the substrate YVAD-pNA. pNA can be measured using a spectrophotometer or a microtiter plate reader at ~405 nm.
      Catalogue Number218790
      Brand Family Calbiochem®
      Product Information
      Detection methodColorimetric
      Form100 Tests
      FormatCuvette or 96-well plate
      Kit containsCell Lysis Buffer, Reaction Buffer, YVAD-pNA Substrate, DTT, Dilution Buffer, and a user protocol.
      Quality LevelMQ100
      Biological Information
      Assay time2.5-3 h
      Sample TypeCell lysates
      Safety Information
      R PhraseR: 36/38-41-52/53

      Irritating to eyes and skin.
      Risk of serious damage to eyes.
      Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.
      S PhraseS: 26-36/39-24-61

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing and eye/face protection.
      Avoid contact with skin.
      Avoid release to the environment. Refer to special instructions/safety data sheet.
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Storage ConditionsUpon arrival store the entire contents of the kit at (-20°C). Store Cell Lysis Buffer, 2X Reaction Buffer, and Dilution Buffer at 4°C after thawing.
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsCell Lysis Buffer, Reaction Buffer, YVAD-pNA Substrate, DTT, Dilution Buffer, and a user protocol.
      Global Trade Item Number
      Catalogue Number GTIN
      218790 0