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71206 Benzonase® Nuclease HC, Purity > 99%

Effective Viscosity Reduction and Removal of Nucleic Acids from protein solutions

Benzonase® Nuclease HC, Purity > 99% MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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71206
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Overview

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      Description
      OverviewBenzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens. It degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity. It is effective over a wide range of conditions and possesses an exceptionally high specific activity. The enzyme completely digests nucleic acids to 5′-monophosphate terminated oligonucleotides 2 to 5 bases in length (below the hybridization limit), which is ideal for removal of nucleic acids from recombinant proteins, enabling compliance with FDA guidelines for nucleic acid contamination. The ability of Benzonase to rapidly hydrolyze nucleic acids makes the enzyme an excellent choice for viscosity reduction to reduce processing time and increase yields of protein. For example, the enzyme is compatible with BugBuster® and PopCulture® Protein Extraction Reagents and can therefore be added along with these reagents to eliminate viscosity and remove nucleic acids from E. coli extracts.

      The enzyme consists of two subunits of 30 kDa each. It is functional between pH 6 and 10 and from 0-42°C and requires 1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF (1 mM), EDTA (1 mM) and urea (relative activity depends on specific conditions). Activity is inhibited by > 150 mM monovalent cations, > 100 mM phosphate, > 100 mM ammonium sulfate, or > 100 mM guanidine HCl.

      Benzonase Nuclease is available in ultrapure (> 99% by SDS-PAGE) and pure (> 90%) grades at a standard concentration of 25-29 U/µl and at a high concentration (HC) of 250 U/µl. Both preparations are free of detectable protease and have specific activity > 1 × 106 U/mg protein. The > 99% purity grade is tested for endotoxins and contains < 0.25 EU/1000 units. The product is supplied as a 0.2 µm filtered solution in 50% glycerol. Store at -20°C.

      Total endotoxin: < 0.25 EU/1,000 units. Purity: > 99% by SDS-PAGE.
      Catalogue Number71206
      Brand Family Novagen®
      References
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity> 99% by SDS-PAGE.
      Physicochemical Information
      ContaminantsTotal endotoxin: < 0.25 EU/1000 units.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      71206-3 04055977271058

      Documentation

      Benzonase® Nuclease HC, Purity > 99% Certificates of Analysis

      TitleLot Number
      71206

      Citations

      Title
    • Lisa Kercher, et al. (2007) Prion protein (PrP) expression differences in microglia and astroglia influence scrapie-induced neurodegeneration in retina and brain of transgenic mice. Journal of Virology 81, 10340-10351.
    • Shogo Kobayashi, et al. (2007) Serine 64 phosphorylation enhances the antiapoptotic function of Mcl-1. Journal of Biological Chemistry 282, 18407-18417.
    • Kentaro Miyazaki, et al. (2006) Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution. Journal of Biological Chemistry 281, 10236-10242.
    • Erumbi S. Rangarajan, et al. (2005) Crystallographic trapping of the glutamyl-CoA thioester intermediate of family I CoA transferases. Journal of Biological Chemistry 280, 42919-42928.
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      User Protocols

      Title
      TB261 Benzonase® Nuclease

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      Categories

      Life Science Research > Protein Sample Preparation > Protein Extraction > Nucleases & Enhancers