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OP102 Anti-von Hippel-Lindau (VHL) Mouse mAb (Ig33)

OP102
  
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      Overview

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~30 kDa von Hippel-Lindau (VHL) protein in prostate, lung, colon, breast, bladder or carcinoma tissue.
      Catalogue NumberOP102
      Brand Family Calbiochem®
      References
      ReferencesCorliss, C.L., et al. 1997.Human Pathology 28, 459.
      Lee, S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 1770.
      Siemeister, G., et al. 1996. Cancer Res. 56, 2299.
      Tsuchiya, H., et al. 1996. Cancer Res. 56, 2881.
      Chen, F., et al. 1995. Cancer Res. 55, 4804.
      Duan, D.R., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 6459.
      Duan, D.R., et al. 1995. Science 269, 1402.
      Iliopoulos, O., et al. 1995. Nat. Med. 1, 822.
      Kishida, T., et al. 1995. Cancer Res. 55, 4544.
      Kley, N., et al. 1995. Glia 15, 297.
      Wizigmann-Voos, S., et al. 1995. Cancer Res. 55, 1358.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Positive controlProstate, kidney, lung, colon, breast, bladder, or thyroid carcinoma tissue
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesOriginal Clone Kibel, A., et al. 1995. Science 269, 1444.
      Key Applications Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (1-2 µg/ml)
      Paraffin Sections (2-4 µg/ml; heat pre-treatment required, see comments)
      Application CommentsPrior to staining formalin-fixed, paraffin-embedded tissue sections, boil the tissue in 1 mM EDTA, pH 8.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant fusion protein consisting of amino acids 1-213 of human VHL
      ImmunogenHuman
      CloneIg33
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
      Global Trade Item Number
      Catalogue Number GTIN
      OP102 0