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ABE1042 Anti-monomethyl Histone H3 Antibody (Lys18)

Detect methylated Histone H3 using this rabbit polyclonal antibody, Anti-monomethyl Histone H3 Antibody (Lys18) validated for use in western blotting & Dot Blot.

Anti-monomethyl Histone H3 Antibody (Lys18) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones UniProt Number: AAA34659
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ABE1042
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, YeastChIP, WB, DBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1042
      DescriptionAnti-monomethyl Histone H3 Antibody (Lys18)
      Alternate Names
      • Yeast (S.cerevisiae) partial histone 3 (H3) gene
      • H3K18me1
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect methylated Histone H3 using this rabbit polyclonal antibody, Anti-monomethyl Histone H3 Antibody (Lys18) validated for use in western blotting & Dot Blot.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Western Blotting
      • Dot Blot
      Application NotesWestern Blotting Analysis: A representative lot detected monomethyl Histone H3 (Lys18) in yeast whole cell extracts, which express wild-type monomethyl Histone H3 (Lys18) and not mutant monomethyl Histone H3 (Lys18Ala) (Prof. F. Howe from the laboratory of Prof. J. Mellor, University of Oxford.).

      Dot Blot Analysis: A representative lot detected monomethyl Histone H3 (Lys18) peptide and not unmodified Histone H3 (Prof. F. Howe from the laboratory of Prof. J. Mellor, University of Oxford.).

      Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated monomethyl Histone H3 (Lys18) in 1.6X 10E7 yeast cell equivalents (Prof. F. Howe from the laboratory of Prof. J. Mellor, University of Oxford.).
      Biological Information
      ImmunogenLinear peptide corresponding to yeast Histone H3 monomethylated at Lys18.
      HostRabbit
      Species Reactivity
      • Human
      • Yeast
      Antibody TypePolyclonal Antibody
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~17 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa acid extract.

      Western Blotting Analysis: 1:1,000 dilution of this antibody detected monomethyl Histone H3 (Lys18) in 10 µg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1042 04053252928741