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MABN527 Anti-iNOS/NOS II, clone 13F5.1 Antibody

This Anti-iNOS/NOS II antibody is validated for use in WB, IH for the detection of iNOS/NOS II.

Anti-iNOS/NOS II, clone 13F5.1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: NOS2, NOS2A UniProt Number: P35228
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MABN527
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCMCulture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMABN527
      DescriptionAnti-iNOS/NOS II, clone 13F5.1 Antibody
      Alternate Names
      • Nitric oxide synthase, inducible
      • Hepatocyte NOS
      • HEP-NOS
      • Inducible NO synthase
      • Inducible NOS
      • iNOS
      • NOS type II
      • Peptidyl-cysteine S-nitrosylase NOS2
      Background InformationiNOS/NOS II, also known as Nitric oxide synthase inducible, Hepatocyte NOS, HEP-NOS, Inducible NO synthase, Inducible NOS, iNOS, NOS type II, Peptidyl-cysteine S-nitrosylase , and encoded by the gene name NOS2 and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2). iNOS/NOS II is expressed in the liver, retina, bone cells and airway epithelial cells of the lung.
      References
      Product Information
      FormatCulture Supernatant
      PresentationMouse monoclonal IgG1λ in buffer containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-iNOS/NOS II antibody is validated for use in WB, IH for the detection of iNOS/NOS II.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected iNOS/NOS II in human cerebral cortex, human cerebellum, and human colon tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human iNOS/NOS II.
      Clone13F5.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1λ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NOS2
      • NOS2A
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~130 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human small intestine tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected iNOS/NOS II in 10 µg of human small Intestine tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN527 04055977121629

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies