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OP10L Sigma-AldrichAnti-c-Myc (Ab-1) Mouse mAb (9E10)

This Anti-c-Myc (Ab-1) Mouse mAb (9E10) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Chromatin IP, Paraffin Sections for the detection of c-Myc (Ab-1).

Anti-c-Myc (Ab-1) Mouse mAb (9E10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

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Overview

Replacement Information

Key Spec Table

Species Reactivity	Host	Antibody Type
H, M, R	M	Monoclonal Antibody

Pricing & Availability

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Description
Overview	Recognizes a ~60-67 kDa c-Myc protein in HL60 cells and lung carcinoma tissue.
Catalogue Number	OP10L
Brand Family	Calbiochem®

References
References	LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144. Cole, M.D. 1986. Ann. Rev. Gen. 20, 361. Nisen, P.D., et al. 1986. Cancer Res. 46, 6217. Nau, M.M., et al. 1985. Nature 318, 69. Persson, H., et al. 1984. Science 225, 687. Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.

Product Information
Form	Lyophilized
Formulation	Lyophilized from a volatile buffer, 100 µg BSA.
Negative control	HT1080 cells
Positive control	HL-60 cells or lung carcinoma
Preservative	None
Quality Level	MQ100

Applications
Application References	Epitope Tagging Munro, S., et al. 1986. Cell 46, 291. Immunoblotting Evan, G.I., et al. 1985. Mol. Cell. Biol. 5, 3610. Chromatin Immunoprecipitation Sanyal, K., et al. 2004. Proc. Natl. Acad. Sci. USA 101, 11374.
Key Applications	Flow Cytometry Frozen Sections Immunoblotting (Western Blotting) Immunofluorescence Immunoprecipitation Not Paraffin Sections
Application Notes	Flow Cytometry (20 µg/ml or use Cat. No. OP10F) Frozen Sections (2-10 µg/ml) Immunoblotting (1-5 µg/ml, see application references) Immunofluorescence (1-5 µg/ml or use Cat. No. OP10F) Immunoprecipitation (1 µg/sample) Chromatin Immunoprecipitation (5 µg/ml, see application references) Paraffin Sections (not recommended)
Application Comments	The c-Myc protein is extremely labile. c-Myc degradation can be as a result of freeze/thaw cycles, thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-Myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. This antibody recognizes the c-Myc protein and its cleavage products. The sequence of c-Myc predicts a ~45 kDa protein, but c-Myc migrates under reducing conditions as a ~64-67 kDa band. This antibody will not detect v-Myc but may react weakly to rodent c-Myc when used at high concentrations (10 µg/ml). An extra ~34-40 kDa band may be detected in a immunoblot. May also be used in immunofluorescence as well as the detection and purification of recombinant proteins tagged with the Myc epitope sequence EQKLISEEDL (see application references). The immunogen, c-Myc (Peptide-1) is also available for competition studies (Cat. No. PP06). Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	a synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) corresponding to amino acids 408-439 of human c-Myc
Immunogen	Human
Epitope	within amino acids 410-419
Clone	9E10
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
OP10L-200UG	04055977225044

Documentation

Anti-c-Myc (Ab-1) Mouse mAb (9E10) MSDS

Title
Safety Data Sheet (SDS)

Anti-c-Myc (Ab-1) Mouse mAb (9E10) Certificates of Analysis

Title	Lot Number
OP10L	Enter Lot Number

References

Reference overview
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144. Cole, M.D. 1986. Ann. Rev. Gen. 20, 361. Nisen, P.D., et al. 1986. Cancer Res. 46, 6217. Nau, M.M., et al. 1985. Nature 318, 69. Persson, H., et al. 1984. Science 225, 687. Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	02-October-2007 RFH
Application	Flow Cytometry (20 µg/ml or use Cat. No. OP10F) Frozen Sections (2-10 µg/ml) Immunoblotting (1-5 µg/ml, see application references) Immunofluorescence (1-5 µg/ml or use Cat. No. OP10F) Immunoprecipitation (1 µg/sample) Chromatin Immunoprecipitation (5 µg/ml, see application references) Paraffin Sections (not recommended)
Description	Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the ~64-67 kDa (apparent MW) c-Myc protein.
Background	The v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc, and B-myc. Amplification of the c-myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-myc gene has been found amplified in neuroblastomas, small cell lung carcinomas and Wilms tumors. L-myc is amplified in small cell lung cancer. Immunological studies have shown that the human c-myc gene gives rise to at least two nuclear phosphoproteins of ~64 kDa and ~67 kDa that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.
Host	Mouse
Immunogen species	Human
Immunogen	a synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) corresponding to amino acids 408-439 of human c-Myc
Epitope	within amino acids 410-419
Clone	9E10
Isotype	IgG₁
Species	human, mouse (weakly), rat (weakly)
Positive control	HL-60 cells or lung carcinoma
Negative control	HT1080 cells
Form	Lyophilized
Formulation	Lyophilized from a volatile buffer, 100 µg BSA.
Preservative	None
Comments	The c-Myc protein is extremely labile. c-Myc degradation can be as a result of freeze/thaw cycles, thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-Myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. This antibody recognizes the c-Myc protein and its cleavage products. The sequence of c-Myc predicts a ~45 kDa protein, but c-Myc migrates under reducing conditions as a ~64-67 kDa band. This antibody will not detect v-Myc but may react weakly to rodent c-Myc when used at high concentrations (10 µg/ml). An extra ~34-40 kDa band may be detected in a immunoblot. May also be used in immunofluorescence as well as the detection and purification of recombinant proteins tagged with the Myc epitope sequence EQKLISEEDL (see application references). The immunogen, c-Myc (Peptide-1) is also available for competition studies (Cat. No. PP06). Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
Toxicity	Standard Handling
References	LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144. Cole, M.D. 1986. Ann. Rev. Gen. 20, 361. Nisen, P.D., et al. 1986. Cancer Res. 46, 6217. Nau, M.M., et al. 1985. Nature 318, 69. Persson, H., et al. 1984. Science 225, 687. Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
Application references	Epitope Tagging Munro, S., et al. 1986. Cell 46, 291. Immunoblotting Evan, G.I., et al. 1985. Mol. Cell. Biol. 5, 3610. Chromatin Immunoprecipitation Sanyal, K., et al. 2004. Proc. Natl. Acad. Sci. USA 101, 11374.

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