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OP17L Anti-c-Fos (Ab-1) Mouse mAb (2G9C3)

This Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of c-Fos (Ab-1).

Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP17L
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Pricing & Availability

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OP17L-200UG
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      Description
      OverviewRecognizes both the ~55 kDa c-Fos and ~62 kDa v-Fos proteins in HeLa cells. Does not cross-react with the ~39 kDa Jun protein.
      Catalogue NumberOP17L
      Brand Family Calbiochem®
      References
      ReferencesJensen, D.E., et al. 1991. Oncogene 6, 1219.
      Reiss, M., et al. 1990. Cancer Res. 50, 6641.
      RouscherIII, F.J., et al. 1988. Science 240, 1010.
      Sassone-Corsi, P., et al. 1988. Cell 54, 553.
      Giardina, S.L., et al. 1987. Anal Biochem. 161, 109.
      Verma, I.M., et al. 1987. Cancer Res. 49, 29.
      Verma, I.M., et al. 1987. Cell 51, 513.
      Muller, R. 1986. Viochem. Biophys. Acta. 823, 207.
      Verma, P. 1986. Trends Genet. 2, 93.
      Greenberg, M., et al. 1984. Nature (London) 311, 433.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 200 µg BSA.
      Positive controlHeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Not Paraffin Sections
      Application NotesImmunoblotting (2.5-5 µg/ml)
      Immunocytochemistry (2.5-5 µg/ml)
      Paraffin Sections (not recommended)
      Application CommentsRecognizes both cellular and viral forms of fos. Does not react with the ~39 kDa jun protein. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (KVEQLSPEEEEKRRIRRERNKMAAA) corresponding to amino acids 128-152 of human c-Fos
      ImmunogenHuman
      Clone2G9C3
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute in sterile PBS, PH 7.4, or sterile Tris-saline (20 mM Tris containing 150 mM NaCl), pH 7.4, to a final concentration of 200 µg/ml. To achieve complete solubilization, reconstitute at (4°C) with 0.1% sodium azide or aliqout and freeze (-20°C) for long-term storage. Note: sodium azide is cytotoxic.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP17L-200UG 04055977208818

      Documentation

      Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) Certificates of Analysis

      TitleLot Number
      OP17L

      References

      Reference overview
      Jensen, D.E., et al. 1991. Oncogene 6, 1219.
      Reiss, M., et al. 1990. Cancer Res. 50, 6641.
      RouscherIII, F.J., et al. 1988. Science 240, 1010.
      Sassone-Corsi, P., et al. 1988. Cell 54, 553.
      Giardina, S.L., et al. 1987. Anal Biochem. 161, 109.
      Verma, I.M., et al. 1987. Cancer Res. 49, 29.
      Verma, I.M., et al. 1987. Cell 51, 513.
      Muller, R. 1986. Viochem. Biophys. Acta. 823, 207.
      Verma, P. 1986. Trends Genet. 2, 93.
      Greenberg, M., et al. 1984. Nature (London) 311, 433.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      ApplicationImmunoblotting (2.5-5 µg/ml)
      Immunocytochemistry (2.5-5 µg/ml)
      Paraffin Sections (not recommended)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with AG.8653X mouse myeloma cells. Recognizes the ~55 kDa c-fos and the ~62 kDa v-fos proteins.
      BackgroundThe proto-oncogene fos has been implicated in cell growth, differentiation, and development. Proto-oncogene fos is induced by a large number of stimuli ranging from mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic peptide (KVEQLSPEEEEKRRIRRERNKMAAA) corresponding to amino acids 128-152 of human c-Fos
      Clone2G9C3
      IsotypeIgG2a
      Specieshuman, mouse, rat
      Positive controlHeLa cells
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 200 µg BSA.
      PreservativeNone
      CommentsRecognizes both cellular and viral forms of fos. Does not react with the ~39 kDa jun protein. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute in sterile PBS, PH 7.4, or sterile Tris-saline (20 mM Tris containing 150 mM NaCl), pH 7.4, to a final concentration of 200 µg/ml. To achieve complete solubilization, reconstitute at (4°C) with 0.1% sodium azide or aliqout and freeze (-20°C) for long-term storage. Note: sodium azide is cytotoxic.
      Toxicity Standard Handling
      ReferencesJensen, D.E., et al. 1991. Oncogene 6, 1219.
      Reiss, M., et al. 1990. Cancer Res. 50, 6641.
      RouscherIII, F.J., et al. 1988. Science 240, 1010.
      Sassone-Corsi, P., et al. 1988. Cell 54, 553.
      Giardina, S.L., et al. 1987. Anal Biochem. 161, 109.
      Verma, I.M., et al. 1987. Cancer Res. 49, 29.
      Verma, I.M., et al. 1987. Cell 51, 513.
      Muller, R. 1986. Viochem. Biophys. Acta. 823, 207.
      Verma, P. 1986. Trends Genet. 2, 93.
      Greenberg, M., et al. 1984. Nature (London) 311, 433.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies