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MABT339 Anti-ZO-1 Antibody, clone 5G6.1

This Anti-ZO-1 Antibody, clone 5G6.1 is validated for use in Western Blotting, Immunocytochemistry, Flow Cytometry, ELISA for the detection of ZO-1 .

Anti-ZO-1 Antibody, clone 5G6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: TJP1, ZO1 UniProt Number: Q07157
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MABT339
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT339
      DescriptionAnti-ZO-1 Antibody, clone 5G6.1
      Alternate Names
      • Tight junction protein ZO-1
      • Tight junction protein 1
      • Zona occludens protein 1
      • Zonula occludens protein 1
      • ZO-1
      Background InformationTight junction protein ZO-1 (UniProt Q07157; also known as Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1) is encoded by the TJP1 gene (also known as ZO1) (Gene ID 7082) in human. The tight junction (TJ) constitutes the barrier between the apical and the basolateral domains of the plasma membrane. The assembly and permeability of this barrier are dependent on the zonula occludens (ZO) membrane-associated guanylate kinase (MAGUK) proteins ZO-1, ZO-2, and ZO-3. ZO-1, a 210-225 kDa protein, is found at the submembranous domain of TJs in epithelia and endothelia. ZO-1 contains three PDZ domains (PDZ1/aa23-109; PDZ2/aa181-261; PDZ3/aa422-502) at its N-treminal end, followed by an SH3 domain (aa519-580), a GUK/GK homology domain (aa632-782), an acidic domain (aa817-894), an alpha spliced domain (aa921-1000), and a C-terminal proline-rich/PR domain. ZO-1 is a phosphoprotein and a known substrate of serine/threonine kinases ZAK and of PKC. MAPK signaling pathway regulates tyrosine phosphorylation of ZO-1, and MEK1 inhibition in Ras transformed epithelial cells is reported to result in tyrosine phosphorylation of ZO-1 and occludin. ZO-1 interacts with claudins, JAM, ZO-2, and ZO-3 through its PDZ domains, while its GK module mediates interaction with occludin. ZO-1 also binds actin cytoskeleton and actin-binding protein 4.1 through its carboxyl terminal end. In addition, ZO-1 associates with AF-6 and cingulin at the TJ, as well as with the adherens junction protein alpha-catenin and with the gap junction proteins connexins 43 and 45.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-ZO-1 Antibody, clone 5G6.1 is validated for use in Western Blotting, Immunocytochemistry, Flow Cytometry, ELISA for the detection of ZO-1 .
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected ZO-1 in human and rat kidney tissues.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human ZO-1.
      Clone5G6.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TJP1
      • ZO1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~195 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HCT116 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected ZO-1 in 200 µg of HCT116 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT339 04055977173505

      Documentation

      Anti-ZO-1 Antibody, clone 5G6.1 MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-ZO-1 Antibody, clone 5G6.1 Certificates of Analysis

      TitleLot Number
      Anti-ZO-1, clone 5G6.1 - 3219908 3219908
      Anti-ZO-1, clone 5G6.1 - 3274285 3274285
      Anti-ZO-1, clone 5G6.1 - 3590146 3590146
      Anti-ZO-1, clone 5G6.1 - 3744303 3744303
      Anti-ZO-1, clone 5G6.1 - 3891833 3891833
      Anti-ZO-1, clone 5G6.1 - 3923541 3923541
      Anti-ZO-1, clone 5G6.1 - 3958817 3958817
      Anti-ZO-1, clone 5G6.1 - 3988493 3988493
      Anti-ZO-1, clone 5G6.1 - 4023307 4023307
      Anti-ZO-1, clone 5G6.1 - 4044216 4044216
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies