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ABE2917-25UL Anti-ZNF189

Anti-ZNF189, Cat. No. ABE2917, is a rabbit polyclonal antibody that detects Zinc finger protein 189 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

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ABE2917-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE2917-25UL
      DescriptionAnti-ZNF189
      Alternate Names
      • Zinc finger protein 189
      • ZFP189
      Background InformationZinc finger protein 189 (UniProt: Q8BKP2; also known as ZNF189, ZFP189) is encoded by the Zfp189 gene (Gene ID: 230162) in murine species. ZFP189 is a highly conserved member of the Krüppel-associated box (KRAB)-containing group of zinc finger proteins and is present in the nucleus of all cell types. It is involved in the regulation of transcription by RNA polymerase II. It contains 16 C2H2 type zinc finger domains and a KRAB domain at its N-terminal (aa 1-75) that is divided into box A and B. Box A is essential for transcriptional repression. Overexpression of Zfp189 gene in prefrontal cortical neurons is shown to preferentially activate gene network that is unique to resilience in mice. CREB is predicted to be its upstream regulator that binds to the zfp189 promoter. (Ref.: Lorsch, ZS et al. (2019). Nature Neurosci. 22(9); 1413-1423; Odeberg, J. et al. (1998). Genomics. 50(2); 213-221).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-ZNF189, Cat. No. ABE2917, is a rabbit polyclonal antibody that detects Zinc finger protein 189 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected ZNF189 in mouse and rat brain tissue lysate.

      Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected ZNF189 in mouse brain tissue sections.

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ZNF189 in mouse nucleus accumbens (Courtesy of Dr. Nestler Lab @ Mount Sinai New York).

      Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected ZNF189 in U2OS cell line.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal half of mouse Zinc finger protein 189.
      EpitopeN-terminal half
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Zinc finger protein 189. It targets an epitope within 14 amino acids from the N-terminal half.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Zfp189
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~70 kDa observed; 70.52 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U2OS cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ZNF189 in U2OS cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE2917-25UL 04065265944878