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CP74 Anti-Vinculin Mouse mAb (V284 or VLN01)

This Anti-Vinculin Mouse mAb (V284 or VLN01) is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Vinculin.

Anti-Vinculin Mouse mAb (V284 or VLN01) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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CP74
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
CP74-100UG
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      Description
      OverviewRecognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins in skin tissue.
      Catalogue NumberCP74
      Brand Family Calbiochem®
      Application Data
      Detection of human vinculin by immunoblotting. Sample: Human vinculin. Primary antibody: Anti-Vinculin Mouse mAb (V284 or VLN01) (Cat. No. CP74) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesPalovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol. 79, 961.
      Bakolitsa, C., et al. 1999 Cell 99, 603.
      Rudiger, M., et al. 1998 Bioessays 20, 733
      Tidball, J.G., et al. 1999 J. Biol. Chem. 274, 33155
      Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil. 17, 1.
      Asijee, G.M., et al. 1990 Eur. J. Biochemistry 189, 131
      Otto, J.J. 1990 Cell Motil. Cytoskeleton 16, 1.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Negative controlNormal mouse serum
      Positive controlSkin cells or tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Paraffin Sections
      Application NotesImmunoblotting (1 µg/ml, chemiluminescence)
      Immunoprecipitation (2 µg/ml lysate)
      Paraffin Sections (4-8 µg/ml, heat pre-treatment required)
      Application CommentsThis antibody is useful for studies of the role of vinculin in platelet activation. Vinculin (Ab-1) exhibits membrane and cytoplasmic staining when used in immunohistochemistry. For staining of formalin-fixed paraffin sections, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogensemi-purified, human vinculin
      ImmunogenHuman
      CloneV284 or VLN01
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Global Trade Item Number
      Catalogue Number GTIN
      CP74-100UG 04055977221022