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PC234L
Sigma-AldrichAnti-Vasopressin Rabbit pAb
This Anti-Vasopressin Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Vasopressin.
More>>This Anti-Vasopressin Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Vasopressin. Less<<
Anti-Vasopressin Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Recognizes vasopressin in rat hypothalamus.
Catalogue Number
PC234L
Brand Family
Calbiochem®
Application Data
Detection of rat vasopressin by immunohistochemistry. Sample: Rat paraventricular nucleus and supraoptic nucleus. Primary antibody: Anti-Vasopressin Rabbit pAb (Cat. No. PC234L) (1:400). Detection: fluorescence.
Antibody specificity was examined in the rat paraventricular nucleus and supraoptic nucleus. Staining is completely eliminated by pretreatment of antibody with arginine vasopressin (10 µg/ml). Preabsorption of antibody with oxytocin (100 µg/ml) resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
rat arginine vasopressin
Immunogen
Rat
Host
Rabbit
Isotype
IgG
Species Reactivity
Rat
Antibody Type
Polyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months. Avoid freeze/thaw cycles of solutions.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Detection of rat vasopressin by immunohistochemistry. Sample: Rat paraventricular nucleus and supraoptic nucleus. Primary antibody: Anti-Vasopressin Rabbit pAb (Cat. No. PC234L) (1:400). Detection: fluorescence.
Description
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes vasopressin.
Background
Neurotransmitters can be defined as chemical substances that are released by the presynaptic neuron before being bound by receptors on the postsynaptic cell surface. It has been well established that the nervous system utilizes neurotransmitters for cell signaling. Neurotransmitters can be divided into two groups; low molecular weight transmitter molecules such as biogenic amines, purines, or amino acids, and bioactive peptides, of which more than 50 members have been identified in the mammalian central nervous system. Many low molecular weight neurotransmitters are produced within the axon terminal from common cellular metabolites and packaged into synaptic vesicles. In contrast, neuropeptides are synthesized in the cell body and packaged into large dense core vesicles for delivery to the presynaptic membrane. The transport and delivery of vesicles containing neurotransmitter is a highly regulated and complex process. The neuropeptide vasopressin plays important roles in vasoconstriction, antinociception, behavior, electrolyte balance, and coagulation functions. Vasopressin helps regulate the hypothalamic-pituitary-adrenal axis along with the corticotropin-releasing hormone. The immuno-localization, neuronal distribution, and vesicular compartmentalization of neurotransmitter molecules and their precursors are important for understanding the modulation, regulation, and function of neurotransmitters in the nervous system.
Host
Rabbit
Immunogen species
Rat
Immunogen
rat arginine vasopressin
Isotype
IgG
Species
rat
Positive control
Rat hypothalamus
Form
Lyophilized
Formulation
Undiluted serum.
Preservative
≤0.1% sodium azide
Comments
Antibody specificity was examined in the rat paraventricular nucleus and supraoptic nucleus. Staining is completely eliminated by pretreatment of antibody with arginine vasopressin (10 µg/ml). Preabsorption of antibody with oxytocin (100 µg/ml) resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months. Avoid freeze/thaw cycles of solutions.
